Campbell Biology
12th Edition
ISBN: 9780135188743
Author: Urry
Publisher: PEARSON
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Question
Chapter 10.4, Problem 5CC
Summary Introduction
To find: The role of glyceraldehyde 3-phosphate (G3P) in glycolysis and Calvin cycle.
Introduction:
Glycolysis is a stepwise process by which one molecule of glucose breaks into two molecules of pyruvic acid by partially oxidizing glucose. This process occurs in the cytoplasm of the cell.
Calvin cycle is a biochemical pathway for the reduction of CO2 or photosynthetic carbon. The acceptor molecule is a 5-carbon ketoses sugar i.e. Ribulose 5-bisphosphate (5 RuBP).
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Chymotrypsin has the highest affinity for which of the following substrates:
Table. The values of KM and kcat for some Enzymes and Substrates
Enzyme
Chymotrypsin
Ки (М)
4.4 x 10-1
8.8 x 10-2
6.6 x 104
Kcat (S-1)
5.1 x 10-2
1.7 x 10-1
1.9 x 102
Substrate
N-acetylglycine ethyl ester
N-acetylvaline ethyl ester
N-acetyltyrosine ethyl ester
Catalase
H2O2
2.5 x 10-2
1.0 x 107
Urease
Urea
2.5 x 10-2
4.0 x 105
OA. N-acetylglycine ethyl ester
OB. N-acetylvaline ethyl ester
OC. N-acetyltyrosine ethyl ester
D. Urea
. Consider the enzymatic reaction scheme: Asparagine + H20 Aspartate + NH3:
a) calculate the specific activity of the enzyme, if in 30 seconds as a result of
a reaction involving 3 mg of the enzyme under optimal conditions (pH 8.0,
37 °C) 75 umol aspartate is obtained;
b) describe the reasons for the decrease in enzyme activity after incubation for
6.
10 minutes at 70 °C (provide an appropriate graph).
Suppose that your Ph.D. mentor asks you to compare the kinetic behavior of the two recently discovered peptidase isozymes PepA and PepB. Your mentor's initial studies identified a pentapeptide substrate called Tide5 that appeared to be able to distinguish the kinetic behaviors of PepA and PepB. All they had time to tell you was this: At low Tide5 concentration, the ?o (initial velocity) of PepA was greater than PepB, but at high Tide5 concentration, the reverse was true.
Before you start your kinetic assays, you decide it would be wise to ponder this small bit of information. You have no numbers to work with, but does it tell you something about the relative values of ?m and ?max for PepA and PepB?
Select the statements that are consistent with the information that you were given.
Chapter 10 Solutions
Campbell Biology
Ch. 10.1 - Prob. 1CCCh. 10.1 - Prob. 2CCCh. 10.2 - Prob. 1CCCh. 10.2 - Prob. 2CCCh. 10.2 - Prob. 3CCCh. 10.3 - Prob. 1CCCh. 10.3 - Prob. 2CCCh. 10.3 - Prob. 3CCCh. 10.4 - Prob. 1CCCh. 10.4 - Prob. 2CC
Ch. 10.4 - Prob. 3CCCh. 10.4 - Prob. 4CCCh. 10.4 - Prob. 5CCCh. 10.5 - Prob. 1CCCh. 10.5 - Prob. 2CCCh. 10.5 - Prob. 3CCCh. 10.5 - Prob. 4CCCh. 10.6 - Prob. 1CCCh. 10 - Prob. 10.1CRCh. 10 - Prob. 10.2CRCh. 10 - Prob. 10.3CRCh. 10 - Prob. 10.4CRCh. 10 - Prob. 10.5CRCh. 10 - Prob. 10.6CRCh. 10 - The light reactions of photosynthesis supply the...Ch. 10 - Which of the following sequences correctly...Ch. 10 - How is photosynthesis similar in C4 plants and CAM...Ch. 10 - Prob. 4TYUCh. 10 - Prob. 5TYUCh. 10 - In mechanism, photophosphorylation is most similar...Ch. 10 - Prob. 7TYUCh. 10 - Prob. 8TYUCh. 10 - EVOLUTION CONNECTION Photorespiration can decrease...Ch. 10 - SCIENTIFIC INQUIRY MAKE CONNECTIONS The following...Ch. 10 - Prob. 11TYUCh. 10 - SYNTHESIZE YOUR KNOWLEDGE Watennelon snow in...
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