The significance of the alteration in the given absorbance of nitrotyrosine after the addition of the substrate analog succinate at 430 nm is to be stated. Concept introduction: The concerted model suggests that subunits of enzymes are arranged in such connection in which any conformational change in any of the subunit is compulsorily granted to all the subunits.

Question

8 of 15 An enzvme has an all-important histidine residue in its active site such that substitution with an other amino acid would result to the loss of catalytic activity. What are the possible mechanisms by which this histidine residue may facilitate catalysis? I. Acting as proton acceptor. II. Forming of covalent bond with the substrate via an ester bond. III. Serve as stabilizer of electrophilic substrate because of its nucleophilic character. Select the correct response: land II Il and III land IlI I. Il, and III

Answer 1

Question

Chapter 10, Problem 33P

Interpretation Introduction

Interpretation:

The significance of the alteration in the given absorbance of nitrotyrosine after the addition of the substrate analog succinate at 430 nm is to be stated.

Concept introduction:

The concerted model suggests that subunits of enzymes are arranged in such connection in which any conformational change in any of the subunit is compulsorily granted to all the subunits.

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