Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Question based on SDS-PAGE
You are working with a 60 kDa protein that you want to visualize on a SDS-PAGE. What % separating gel will you use in your SDS-PAGE?
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- 2.7 Problem in the Determination of Molecular Welght by Gel Electrophoresis A puri- fied recombinant protein is analyzed for molecular weight by SDS-PAGE at pH 8.5. From the protein sequence deduced from the gene that was expressed in bacteria, the protein is expected to have a molecular weight of 44,000. However, the molecular weight of the pro- tein is found by SDS-PAGE to be 52.000. Explain the reason or reasons for this difference in molecular weight. What calculation could you make to help explain this discrepancy?arrow_forwardFill up correct options.arrow_forwardPlease find all 5 mistakes and provide corrections for all 5 mistakesarrow_forward
- Protein sequences which are usually more advantageous than DNA sequence in the comparative pairwise alignment analysis. Briefly explain and elaborate this statement.arrow_forwardstep by step calculations pleasearrow_forwardNo plagiarism i will double check (I only accept hand written answers thank you) i will rate helpfularrow_forward
- Problem 4 DNA Sequence. Suppose that you perform shotgun sequencing and found that you got the following subsequence {CAT, TCA, AAG, GCT, GCA, ATC, CAA, AGC} What is the original sequence?arrow_forwardIn Multi-Column Purification of rGFP. What happens to the protein amount, protein purity, and/or specific activity of a purification fraction if one of the three is changed? (i.e. understand the relationship between the three.)arrow_forwardTell the correct option onlyarrow_forward
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