3) Suppose the major solutes in intact lysosomes are KCI (-0.1 M) and NaCl (-0.03 M). When isolating lysosomes, what concentration of sucrose is required in the extracting solution at room temperature (25°C) to prevent swelling and lysis? 4) The following questions will help you review and understand pH, pKa, and buffering by weak acids/bases. a) Water is a weak acid; write the ionization reaction of water and the expression that describes its K. (Hint: K, is the equilibrium constant for proton dissociation). b) What is the concentration of H* in a solution of water when the concentration of OH = 108 M? What is the pOH of this solution? What is the pH? Is the solution acidic or basic? c) Draw a titration curve for a molecule with a pKa = 4.7 and label all the axis, indicating clearly the buffering region and the pKa value. d) Calculate the fraction of histidine (amino acid shown below), that has its imidazole side chain protonated at pH 6.4. The pK, value for the imidazole group of histidine is 6. Protein CH₂ Protein NH NH pH5 pH 7 CH+H

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Question 2. An enzyme was found to convert Acetyl-coA to an acetyl-cysteine intermediate of the enzyme. The structure of cOA is: H H НО СН; HS — СH2— СH2 — N—C—CH,— CH2—N- С—С—С—CH;—0—Р- 0–CH2 Adenine н CH H H H Phosphopantetheine group of coenzyme A 2-O3PO ОН Draw a plausible chemical mechanism. Where is the acetyl-group in acetyl-coA? What is the reactive group of the protein? The reaction is enhanced at higher pH. How is this consistent with your mechanism? Is covalent catalysis involved?

Suggest a reason why trans fatty acids have melting points similar to analogous saturated fatty acids.

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Question 3 of 3 > r: te e Select the true statements about protein secondary structure. IS IS In a B-pleated sheet, the side chains are located between adjacent segments. Peptide bonds stabilize secondary structure. The secondary level of protein structure refers to the spatial arrangements of short segments of the protein. In an a-helix, the side chains are located on the outside of the helix. The a-helix is held together by hydrogen bonds between the amide N-H and C=0 groups. earning, Inc. privacy policy terms of use contact us help about us careers 112 30 FEB 2 4.

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Answer questions for the following monosaccharide: H-C-OH НО —с —н H C-OH d. Draw the product if it were oxidized ([O]). Draw entire product. H -C-OH e. Convert & draw the beta anomer (Haworth structure). CH2OH