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Write the equation for the bicarbonate buffer. What enzyme catalyzes this reaction?
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- Write the equations for reaction of this buffer with a small amount of HNO3 and with a small amount of NaOH.In the biological fluids, such as blood, name two biomolecules that can act as the buffering (1) (f) components. Explain how this is achieved.How is Ka defined? Write the equation for Ka for the generalized acid HA.
- Consider the following pH titration curve of a diprotic acid. What is the approximate values for pka 1 and pka 2? the curve is attached below.Write the enzyme equation for catalase and hydrogen peroxide.The glass electrode used in commercial pH meters gives an electrical response proportional to the concentration of hydrogen ion. To convert these responses to a pH reading, the electrode must be calibrated against standard solutions of known H+ concentration. Determine the weight in grams of sodium dihydrogen phosphate (NaH2PO4 · H2O; FW 138) and disodium hydrogen phosphate (Na2HPO4 ; FW 142) needed to prepare 1 L of a standard buffer at pH 7.00 with a total phosphate concentration of 0.100 M . See Problem 24 for the pKa values of phosphoric acid.
- Given the following information, calculate the total activity in the undiluted protein sample. Activity of 1 ml of diluted sample = 0.5 Total volume of sample = 5 ml Dilution factor = 10 25 50.5 250 2.5An amylase result is 550 U/L. A 1:4 dilution is made and reads 180 U/L. What is the reported result?You have been provided with stock solutions of: stock A: 0.06 M sodium pyrophosphate buffer pH 8.5 stock B: 3 M ethanol stock C: 0.015 M NAD+ stock D: milli Q water Determine the volume you will need of each solution to prepare a buffer of with a final volume of 60 mL containing 10 mM sodium pyrophosphate pH 8.5, 100 mM ethanol, 1 mM NAD+. i.e. volume of stock A = _________mL volume of stock B = _________mL volume of stock C = _________mL volume of stock D = _________mL Show your calculations to arrive at your answers.