With respect to an intron, which one of the following options would be a good way to identify the location of the branchpoint? A located 15-45 bases from 3’ splice junction GU located at the 5’ splice site AG located at the 3’ splice site AAUAAA near the 3’ UTR

IX. Dibuje la estructura del polinucleótido que aparece a continuación el cual corresponde a un codón en el mRNA que codifica para el aminoácido prolina. (Solamente se pide el dibujo de la estructura siguiendo el modelo de polinucleótido mostrado en clase). e). .) 5'-C-C-C-3' Show Transcribed Text IX. Draw the structure of the polynucleotide below which corresponds to a codon in the mRNA that codes for the amino acid proline. (Only a drawing of the structure following the polynucleotide model is requested)

1. (a) By binding one L-tryptophan molecule/monomer, the trp repressor binds to DNA to sup- press synthesis of L-tryptophan in E. coli. Below is the amino acid sequence of the helix - reverse turn - helix region of the trp repressor that binds to DNA compared to the sequence of the corresponding DNA binding motif of the Prl protein. A diagram of the trp repressor dimer is also shown. Trp Prl Trp Prl 80 -Gly-Glu-Met-Ser-Gln-Arg-Glu-Leu-Lys-Asn-Glu-Leu-Gly-Ala-Gly-Ile- -Ser-Glu-Glu-Ala-Lys-Glu-Glu-Leu-Ala-Lys-Lys-Cys-Gly-Ile-Thr-Val- trp helix 5 70 trp helix 4 Prl helix 80 Prl helix Ala-Thr-Ile-Thr-Arg-Gly-Ser-Asn-Ser-Leu-Lys-Ala-Ala- Ser-Gln-Val-Ser-Asn-Trp-Phe-Gly-Asn-Lys-Arg-Ile-Arg- reverse turn 90 Comparing the two protein sequences above, identify all amino acid pairs that differ in electrostatic charge due to proton dissociable groups (assume pH 7). Indicate the charge of both residues for each such pair. (b) Circle the pair of residues for which the electrostatic charge due to…

For each of the following initiation factors, how would eukaryotic initiation of translation be affected if it were missing? A. eIF 2 B. eIF4 C. eIF5

Sequence most commonly found at the boundary between exon-intron boundaries is            at the 5' end and            at the 3' end     Question 33 options:   G/GU; AG/G   AG/G; G/GU   A/GG; GG/U   All of the above

A novel, RNA-containing splicing factor is purified on the basis of its physical interaction with a previously known subunit of the spliceosome. You sequence the RNA within the new factor, and identify a region that is perfectly complementary to a stretch of the RNA from the previously known subunit with which it interacts. You suspect that the interaction between the new factor and the subunit is mediated by hybridization between these complementary RNA sequences, but would like to be sure. How could you test this experimentally?