Could you explain the difference ( and possibly show the difference) between a growth on Agar slants and Growth in broths? Next, could you describe what E. Coli, M. Luteus, and an uninoculated growth should look like on both agar slants and broths? Much thanks! I am a bit confused

Could you explain the difference ( and possibly show the difference) between a growth on Agar slants and Growth in broths? Next, could you describe what E. Coli, M. Luteus, and an uninoculated growth should look like on both agar slants and broths? Much thanks! I am a bit confused

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter7: Basic Clinical Microbiology

Section7.5: Tests For Group A Streptococcus

Problem 5RQ

See similar textbooks

Related questions

Q: What are the primary limitations imposed by using Hanging-drop method (or Wet mount) to observe…

A: Hanging drop preparation is a type of wet mount in which a drop of organism-containing media is…

Q: You will appreciate the abundance of soil microbes when you consider that the plate of them we…

A: Microbes have a crucial part in the soil's production. They generate lytic enzymes that aid in the…

Q: In a bacterial culture on an agar plate, what is a zone of inhibition? An area where the…

A: Microbial culture is a method of multiplying microbial organisms by letting them reproduce in…

Q: Suppose you are checking the microbial quality of a raw material using agar culture media. After…

A: Unwanted microbial growth occurs when no proper care is taken while tranferring the culture on the…

Q: A specimen transferred onto a fresh agar plate is initially in which phase of bacterial growth?

Q: A bioreactor which is used for the production of growth factor A is operated as fed- batch culture.…

A: A Bioreactor is a device or a process system which supports and habitats the biologically active…

Q: Gram stain of Nodule Suspension Describe the morphology of the crushed nodules under microscopes…

A: The bacteria are stained with the gram stain and can be divided into two groups on the basis of the…

Q: While working with Mannitol Salt Agar you notice the growth of staphylococci however other…

A: Mannitol Salt Agar (MSA) is a media which has high concentration of salt solution. There are found…

Q: If lysozyme is added to a microbial culture. Predict what will be observed if the culture is…

A: Microorganisms are small organism that cannot be seen by naked eyes. They include bacteria, fungi,…

Q: Which of the following would you most expect to find in a fecal contaminated water sample?…

A: Water pollution is described as the fouling of bodies of water. Water pollution occurs when rivers,…

Q: Bacteria are not used as probiotic agents. True O False The growth of bacteria that form endospores…

A: Bacteria are prokaryotic microorganisms. Bacteria are ubiquitous in nature and are found almost in…

Q: A. Observation of growth characteristics on selective and differential media Mannitol salt Agar…

A: S.aureus stands for staphylococcus aureus. If we are to grow this bacteria in Mannitol salt agar…

Q: attern used to inoculate agar slant to get luxuriant culture. This pattern increases the surface…

A: Slant culture : A culture made on the slanting surface of a solidified medium in a test tube that…

Q: MSA/mannitol salt agar plate is selective because: O it allows only gram positive bacteria to grow O…

A: Mannitol salt agar(MSA) It is a selective medium used for the isolation, enumeration and…

Q: a) explain in your own words how to perform specialized media tests to find an unknown bacteria

A: Different bacteria can be identified using the macroscopic properties of microbial cultures. The…

Q: What is bacterial agar?

A: MICROBIAL GROWTH MEDIA- It is the culture media that serves as a nutrient media for the growth of a…

Q: The term psychrophile? a. refers to an organism that has an optimum growth within the range of -5…

A: Bacteria A unicellular prokaryotic microorganisms not visible to the naked eyes.

Q: What type of agar would you use to grow gram positive bacteria

A: Bacteria are different from the plant as well as animals. They require specific substances in the…

Q: If the sterile glycerol stock is 40%, what will be the volume of the bacterial culture in a nutrient…

A: You can store bacteria at 4 °C on an LB agar plate. But, this is suitable only when you want to…

Q: Why do you think it is important to end the tempeh fermentation before the molds sporulate?

A: Tempeh is a traditional dish of he Indonesian people. Tempeh is prepared by fermenting of soybeans…

Q: Which lactose (+), Gram negative, short rod forms a pink to brick red colony on MacConkey's agar and…

A: Bacteria that cannot retain the crystal violet stain as their cell envelopes are composed of…

Q: why is it important that a small amount of inoculum be used in preparation of an agar slant

A: Agar is a jellylike substance derived from the purified cell walls of red algae. It is added to…

Q: Using the Table above as a guide, indicating what media could be used to differentiate the…

A: Introduction : The bacterium Staphylococcus epidermidis is gram-positive. It is a natural component…

Q: (a) You received three cultures (Escherichia coli, Saccharomyces cereviciae, and Rhizopus oryzae)…

A: PCR is used to amplify a target DNA.When boiling method and Gene releaser were used then the…

Q: Compare the growth of the cultures found in Plates A, B, C and D. 1.Which among the four plates has…

A: Microorganisms are minute cells not visible to the naked eye that exists either in a colony or as a…

Q: Why is nitrification a good example of a commensalistic process?

A: The ‘bacteria’ is a microscopic ‘single-cell’ organism. It does not have a nucleus and other…

Q: A meteorite lands at your feet. You remove 4 equal weight samples from the uncontaminated interior…

A: Introduction : L glucose and D- glucose differ in their structures at the chiral carbon. L- glucose…

Q: If one is trying to determine if a culture is pure or mixed, which medium would you choose? Why?…

A: In microbiology, to study the different types of microorganism we use different mediums which are…

Q: During media preparation, you observed that the agar medium did not solidify after sterilization…

A: Nutrient Agar Medium is the artificial media which is used to grow microbes. It provides essential…

Q: Shown below is the growth of E. coli in nutrient agar plates after exposure to different doses of UV…

A: E.coli or Escherichia coli, is a rod shaped, coliform, gram negative bacteria which is facultative…

Q: f a scientist were trying to separate Klebsiella, Proteus, Shigella, and Vibrio into two separate…

A: Let us make a table to analyze their features and then decide which basis of classification will…

Q: How is Leather put in an application in microbiology and its involved microorganism

A: Leather is the skin of cattles and animals used to protect human body.

Q: What is growth in "pure culture"? O Growth of human pathogens Growth of just one species in the lab…

A: A microbial culture is a method of multiplying microbial organisms by letting them reproduce in a…

Q: ow can one be sure that the culture media are sterile or free from contamination

A: Sterility, often known as sterility, is the state of being free of all contaminants and…

Q: Shown below is the growth of E. coli in nutrient agar plates after exposure to different doses of UV…

A: Answer :- The given data infer the effect of increasing UV exposure time on E. coli. With an…

Q: What is the major difference between an enrichment culture and a selective culture?

A: Culture media is used for cultivating microorganisms. Both selective media and enriched media are…

Q: Colonies have grown in a nutrient agar plate. However, it did not grow along the lines of streaking.…

A: Streaking method This method is used to produce isolated colonies of bacteria. This helps in…

Q: Technique used to inoculate plate media using inoculating loop. Technique used to inoculate agar…

A: # Here I am giving solution of first two questions . Please repost your third question and better to…

Q: How do you prepare a Potato Dextrose agar in a 500ml glass bottle (NOT FROM COMMERCIAL POWDER)

A: Potato Dextrose Agar, also called as PDA, is a typical microbial growth medium produced using an…

Q: What type of agar would you use to grow gram negative bacteria

A: Gram-negative bacteria are the bacteria that do not consist of the crystal violet stain and they are…

Q: what information about the identity and physiology of a bacterium or yeast can be learned from…

A: Micro organisms are grown in a solid medium (agar) with supplements as a primary step in order to…

Q: Why does uninoculated agar plates still has visible growth of microorganisms? Where do you think the…

A: Microorganisms are present in abundance in nature. When there are no aseptic conditions then…

Q: How could a mold like Penicillium be protective against certain contaminants but not others?

A: Penicillium is an important genus of phylum ascomycota, found in the natural environment as well as…

Q: A pure culture of an unknown bacterium was streaked onto plates of a variety of media. You notice…

A: A culture medium is a medium that is used to provide support to the growing microorganisms. This…

Q: Should cheesemakers be worried about endospore growth of Gram-positive cells? Why or why not?

A: Bacteria are organisms that are found almost everywhere on earth and are free-living cells that are…

Q: Assume that you had plated your +PGLO culture on an LB agar plate. What result (number of colonies)…

A: +pGLO culture is a type of bacteria that has been genetically modified to produce a fluorescent…

Q: Using your fingers, you are asked to aseptically touch the surface of a sterile agar plate.…

A: The aseptic technique of plating on an agar plate means that a sterile or no infection-containing…

Q: You are given a culture medium on which only Gram negative bacteria will grow. Organisms that are…

A: The medium in which only gram negative bacteria will grow prevents gram positive bacteria to grow in…

Q: main goal of motility agar

A: MICROBIAL GROWTH MEDIA- It is the culture media that serves as a nutrient media for the growth of a…

Concept explainers

Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.

Question

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

This is a popular solution!

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Step 2

VIEW

Step 3

VIEW

Trending now

This is a popular solution!

Step by step

Solved in 3 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.

Similar questions

1) would you describe the contents of the soil-inoculated broth as being a “pure culture”? Why or why not? 2) How did the uninoculated broth differ in appearance from the broths inoculated with E. Coli and M. Luteus? And then how could you tell if a supposedly sterile, uninoculated broth was contaminated? Please explain in detail and highlight the important parts cuz I am confused and need help! Thanks
1(a) 1. What is the theory upon which intermittent sterilization is based? (B) Is dry or moist heat more efficient as a sterilizing agent? Why? (C) When would it be necessary to use aseptic techniques when inoculating a tube of nutrient broth with dirt? (D)Why do some microorganisms characteristically form a growth of the pellicle type? What environmental factors might alter the formation of a pellicle? (This is not a graded assignment)
What type of agar is MacConkey agar?
In disc diffusion studies, where will the lowest concentration of the tested chemical be in the agar plate? in the agar directly below the disc O in the agar halfway between the disc and the petri dish wall O in the agar furthest away from the disc the cehmical is equally distributed throughout the agar
A culture consisting of 100 litres of nutrient medium containing 12 g l-1 of growth limiting substrate is inoculated with 10g of bacteria in a batch fermenter. The yield of biomass from substrate is 0.05 g g-1 . The maintenance coefficient is 2.0 g g-1 h -1 . The specific rate of product formation due to maintenance is 1.0 h-1 . The maximum specific growth rate of the organism is 0.3 h -1 . YPX= 7.7 and product formation is directly linked with metabolism. a) What is the time required to produce 15 g of biomass? !!!!! ANS IS 3.07 HRS!!!! STEPS on how to reach this please
What is the major difference between Thayer-Martin and chocolate agar? When would you use Thayer-Martin rather than chocolate agar?
After inoculating and incubating an agar slant from a pure broth culture of a bacterial species such as E. coli, which of the following would indicate an unsuccessful aseptic transfer? (Choose ALL that apply) a - There is fungal growth in the original broth culture tube. b- There is too much growth on the agar slant. c- There are colonies of similar morphology on the slant. d - There are red, yellow, and white colonies on the slant. e - There is no growth on the slant.
Why is the colony diameter of the yeast culture grown on potato dextrose agar is larger than those grown on acetate agar? Give and discuss the reasons for this different in growth.
Describe how a Triple Sugar Iron agar tube would appear if the following situation was true. Please note that all items in the following list are occurring in the same tube of agar. 1. Positive for glucose fermentation 2. Negative for lactose and sucrose fermentation 3. Positive for H2S production 4. Negative for gas production
You take two stainless steel pins and place each in a separate pan of boiling water for 15 minutes. Using sterile forceps, you then remove each pin from the boiling water and place each separately in a tube of sterile nutrient broth. You put the two tubes of broth in a 35-degree Celsius icubator for two days and then observe them for miucrobial growth. In one you observe extensive growth as evidenced by heavy turbidity, but the other tube remains completely clear, indicating that no growth occurred. Explain.
1. You were given a mixed nutrient agar broth culture of bacteria. a. How will you determine the different types of bacteria present in the mixed culture? b. How will you make a pure culture of these bacteria in a slant nutrient agar? c. How will you identify these bacteria?
10. Which area on a streak plate will contain the greatest amount of growth? The least amount of growth? Explain your answers. 11. What is a colony (as viewed on an agar plate)? How can a pure culture be obtained from a mixture of bacteria? 12. What is a selective agar? Why can it be of importance?