Which plate could represent the results of the transformation experiment positive control plated on LB if the transformation procedure was too harsh on the bacterial cells? Refer to the letters on top of the image. Ignore the letters at the bottom. A B А B C O A O none of the choices are correct

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Experiment 1: An RT-PCR was run on human cells growing in tissue culture under standard conditions, using the primers described above. A normal PCR was also run on the cells, using the same primers. They were run on an agarose gel shown on the left in the figure (gel 1), in the wells indicated by ‘RT-PCR’ and ‘PCR’. The arrow shows the direction of current in the gel. a) Observe the gel and describe the results for the RT-PCR and PCR (no explanation, just describe). b) Explain why the bands have travelled different distances on the gel.

You have conducted a transposon mutagenesis experiment using the same PRL27 system that was used during your lab exercise. After allowing for conjugation. you plate the conjugation mix on Luria agar and Luria + Kanamycin agar. After incubation, you count the following number of colonies on each type of plate: Media Dilution Number of Colonies Too Many to Luria 10-5 Count 10-6 173 10-7 28 Luria + Kanamycin 100 324 10-1 217 10-2 3 Determine the transformation efficiency. Note: You can enter you answer using long form (e.g. 1500000) or scientific notation using e notation (e.g. 1.5e6). Do not enter units.