When a company changes from the LAL assay to the Recombinant Factor C assay, they must perform a study to demonstrate that the two methods give comparable results.  true or false?

You are performing an experiment using CRISPR-cas9 to genetically modify the LacZ gene of a culture of E. coli. After you run the experiment, you decide to use gel electrophoresis to genotype the different bacterial cultures to determine if the gene editing was successful.  How could your electrophoresis results confirm that the PCR was successful? And how could your electrophoresis results confirm that you successfully extracted genomic DNA from your bacterial samples?

Restriction enzymes and DNA ligase are key components when preparing cloning vectors. True or False.

Tailed primer can have restriction enzyme sites. True False

What are the main differences between the two testing methods: short tandem repeat (STR) and restriction fragment length polymorphism (RFLP) (ie time, expense, sample needed, etc)?

When cloning a piece of DNA, the purpose of using the LacZ blue-white colony method is to A) Remove bacteria that do not have recombinant vectors B) Remove bacteria that do not have the DNA insert of interest Remove linear DNA Distinguish colonies that have recombinant vectors from those with non-recombinant vectors. E) Remove bacteria that have not taken up the vector

Describe the steps of the chromatin immunoprecipitation assay.   What are some of the scientific facts that can be learned through a chromatin immunoprecipitation assay?

What is a recombinant microorganism? Discuss examples.

An individual is heterozygous for an allele. Allele A has a SNP that results in disruption of an Not1 restriction enzyme site. Allele B has a functional Not1  restriction enzyme site. PCR is performed on the region of the gene containing the SNP to produce a 255bp PCR product. Restriction digestion was subsequently performed using EcoR1 restriction enzyme and the fragments were electrophoresed on an agarose gel. (EcoR1 cuts allele B at a site 115bp from the end of the sequence.) What are the sizes of the subsequent DNA fragments? 1) 115bp + 140bp 2) 205bp + 255bp 3) 140bp 4) 205bp + 255bp + 115bp 5) 140bp + 205bp + 255bp 6) 255bp + 115bp + 140bp 7) 115bp 8) 255bp 9) 115bp + 255bp 10) 255bp + 115bp + 140bp + 205bp 11) 140bp + 205bp 12) 205bp 13) 205bp + 115bp 14) 140bp + 255bp

All of the following pieces of information are expected to be obtained directly from Western Blot analysis, except: An estimation of the fusion protein’s size Relative quantity of the fusion protein compared to other samples The amino acid sequence of the fusion protein If the fusion protein is present in the sample