Which of these are a DNA sequence are which are a protein? Column A Column B 1. A gene's promoter a. DNA RNA Polymerase b. Protein 3. Introns 4. An exon Transcription factors 6. The start codon 7. The product from a coding gene 8. The transcription start site 2. 5.

Scientists can put spider genes into yeast. The yeast can decode the spider genes to make spider silk protein. Why is this so? Evaluate the models. MODEL 1 Spider DNA mRNA mRNA Spider Protein 3. The yeast used machinery from a spider to read the spider genetic code, so both organisms make a protein that is identical. 1. The yeast transcription machine cannot read the spider gene, so yeast uses a machine from a spider to make an mRNA copy. Transcription Machine 2. The yeast translation machine cannot read the mRNA, so yeast uses a machine from a spider to make the protein. ◄ Translation Machine MODEL 2 Spider DNA ▼ mRNA mRNA Spider Protein 3. Yeast and spiders use the same genetic code, so both organisms make a protein that is identical. 2. Explain why one model is correct and the other one isn't. 1. Which model correctly explains what happens inside the yeast cell? 1. The yeast transcription machine reads the spider gene and makes an mRNA copy. Transcription Machine 2. The yeast…

The RNA polymerase that requires an input of energy to initiate the process of unwinding DNA is  Group of answer choices a.RNA Polymerase I and II b.RNA Polymerase I c.RNA Polymerase II d.RNA Polymerase III e.RNA Polymerase I and III

II. Use the eukaryotic gene DNA sequence below to answer the following questions: 1 11 21 CGACTTACTG 51 TGGACGCGCC 101 61 GGCGTATAAA GCGACGACTG TAGACTGATG AGCCTATCCA 111 71 121 31 ATGGCCCTGT AAGCGGTGCG ATGCAATAAA ACGCGTATCA 81 Polyadenylation signal in the corresponding mRNA: Kozak's sequence in the corresponding mRNA: Start (initiation) codon in the corresponding mRNA: Stop (termination) codon in the corresponding mRNA: 61-69 c. Where is the 3' UTR? Circle one. 70-111 14-60 131 41 GTCATTCAGC GTAGTCTGAT GCCAGTCGAC TGCATTGGAC ACCGGTTACA 91 a. Write the corresponding sequences, circle & label them in the sequence above: TATA box sequence: (label as TATA) 37-45 73-90 141 (label as poly-A) b. What region of mRNA contains the open reading frame that will be translated into protein? Circle one. 51-111 (label as Kozak) (label as start) (label as stop) 71-81 85-150

Identify the sites of lagging strand synthesis. Select all that are true. 52 U a. C b. D c. B. O d. A Next page

Which RNA polymerase produces mRNA? 1. polymerase 3 2. Polymerase 1 3. Polymerase 2 & 3 4. Polymerase 2

6. Similar to the class notes (Intro to Genetics), a segment of DNA (shown below) contains a promoter segment (the first 9 base pairs), a ribosome binding segment (the next 6 base pairs), and a segment that codes for protein synthesis which is started by the rest of the base pairs. ACTCCATTGAACCATTTCTATGATCCGCTAACG-... TGAGGTAACTTGGTAAAGATACTAGGCGATTGC-... A. When the DNA is induced to be copied to mRNA, the top strand is coding, meaning that the mRNA makes an identical copy of the lower strand (replacing T with U) The mRNA copy starts with the ribosome binding sequence. What is the sequence of the mRNA that will go to the ribosomes? B. What are the first 6 amino acids of the protein that are coded for by the mRNA? C. What would the amino acid sequence be if... i. a transition mutation occurred on the final G in the mRNA? ii. all of the G & C bases in the protein synthesis portion had transition mutations? iii. a point deletion mutation occurred in the ATA sequence (in the lower strand…

10. Which statement is true about gene transcription? A. Every gene that is present in the genome is converted into a protein B. Only one of the two DNA strands is transcribed C. Every gene is transcribed to the same degree (makes the same amount of gene product) D. Transcription is different on the leading and on the lagging strand

1.The Shine Dalgarno sequence is ____________. located in the 50S subunit of ribosome. located on the 16S rRNA component of the 30S subunit of ribosome. a sequence upstream of the AUG initiation codon on mRNA. a DNA consensus sequence in the promoter region of a gene. a signal for transcription termination.

1. For each of the items below, give a brief description (indicate function for enzymes) and indicate in which process (replication, transcription, or translation) it is involved with. helicase Description/Funetion catalyzes the unwinding of DNA strands Process involved in replication 11. terminator 12. ribosome 13. amino-acyl RNA site 14. MRNA 15. tRNA 16. rRNA 17. Shine-Dalgarno sequence 18. start codon 19. Okazaki fragments 20. coding strand 21. peptidyl tRNA site