Which of the following statements regarding ionizing radiation is FALSE?   Question options:   pyrimidine dimers are caused by ionizing radiation   x-rays are a form of ionizing radiation   gamma rays are a form of ionizing radiation   electrons are energized by ionizing radiation   chromosomal breaks can result from ionizing radiation   ALL of these statements are TRUE   Question 7     ________ is the first repair system reviewing newly synthesized DNA, and is performed by DNA polymerase III itself.   Question options:   Mismatch repair   Proofreading   SOS response   Dark repair   Light repair with photolyase   Base-excision repair

Instructure.com/courses/47420/quizzes/225324/take missour Question 2. It is your first week working in the lab but unfortunately you program the PCR machine incorrectly and some of your PCR experiments do not work as expected. For each of the following scenarios, state at which step the PCR went wrong, what was wrong and what would have happened. Cycles Which step is incorrect? Is the temp too high or too low? Experiment of What happened? PCR Step 1 94 °C Step 94°C V [ Select ] [ Select ] [ Select ] a step 2 Step 3 72 °C step 3 step 1 Step 1 30 °C Step 2 60 °C [ Select ) [ Select ] [ Select ] Step 3 72 °C Step 1 94 °C [ Select] Step 2 22°C [ Select ] [ Select ] Step 3 72 °C 8 A átv

Question #3: CRISPR has been used to cure an individual from sickle cell. Below is a Sanger electropherogram of a sequence from a patient without sickle cell and one with sickle cell. Sequence from a normal individual mmmm Sequence from the diseased individual G T GIIC A GC A Se SCIENCEphe A G A SCIENCE SCIENCEphoto G a) Where is the change in the sequence and what is the consequence to the protein sequence of this mutation? b) Below is an image of the normal and diseased quaternary hemoglobin protein. What is different about the protein shape and why does that structure have a huge impact on its function (please name the function!)? Adult haemogBRAR G G G G A G Sickle Cell haemoglobin S Structure a s RARY COLIBRARY c) If you were to use CRISPR to modify the genome of a diseased individual, to which nucleotides might you design your guide RNA? Why? d) RNA Seq is used to determine off-target effects of Cas9 cleavage. Why is this an appropriate tool to determine these effects? e) Data on…

Question 2: Mutations - Create a nonsense point mutation in this coding region of the gene. Make sure to highlight the mutation you made using a different color or highlighter.   DNA sense ATG AAA CGA GTT ACC GAA ACT TAA   DNA nonsense                   mRNA codon                   tRNA anticodon                   Amino acid