A 2000 nt plasmid with 10 negative supercoils has L= 210 200 190 400

In Figure 5-19, how many different bacterial species areshown as having contributed DNA to the plasmid pk214?

Margaret has been given a plasmid containing her favorite gene, afg (Margaret’s favorite gene). The only thing sheknows is that afg was cloned into the vector using a single restriction enzyme, BstBI. Margaret orders theBstBI enzyme from NEB, so she can cut the plasmid and confirm the presence of the insert. On theNanodrop, she measures the concentration of her plasmid to be 401 ng/μL. Describe how she would setup and perform this reaction if she wanted to digest 2.0 μg of the plasmid.

Consider the image below. This image shows plasmid DNA isolated through exactly the same method that you used. The lanes that were untreated (TE) contain plasmid DNA, but also large amounts of smaller nucleic acids that are predicted to be RNA. What is the experimental evidence from the gel itself that these smaller molecules are RNA? (Hint: look at the treatments performed in each lane)