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- BOTTOM The sequence depicted on the gel is 5'-GTGATGTAG-3' The sequence depicted on the gel is 3'- GTGATGTAG -5' The sequence shown on the gel is the sequence of the template strand of the reaction The sequence shown on the gel is complementary to the template strand of the reaction The template strand of the reaction is antiparallel to the sequence shown on the gel The largest fragment shown on the gel is closest to the top The largest fragment shown on the gel is closest to the bottom Each of the fragments shown on the gel have a primer incorporated at their 5' end Each of the fragments shown on the gel have a primer incorporated at their 3' end The number of nucleotides in the shortest band on the gel is 21 The number of nucleotides in the longest band on the gel is 21 ick Save and Submit to save and submit. Cick Save All Answers to save all answers.What is the relationship between fluorescence intensity of a spot and the amount of DNA in a sample for a Virochip DNA microassay? From the following which is the best choice Less DNA results in greater fluorescene since the laser passes through he sample eaiser More DNA equals to more intense fluorescence since the DNA makes a layer to refelct the laser light More DNA equals to more intense fluorescence since of more dye on the DNA is fluorescene More DNA results in to more fluorescence since there's more DNA to be excted by the laser None of the aboveUsing the table below, what volume from the master mix will be dispensed to each sample tube if the genomic DNA is 1 µL and the Taq polymerase is 0.3 µL?
- How is the color of the spot coverted into useful data if data is collected from a Vicrochip DNA microarray that's from the colors of spots that illuminate when the spots have a laser shine on them? From the following which one is the best option The color of the spot is converted to a number that represents the intensity of green or red, so that the numerical intensity values can be compared between spots by a computer program The color of the spot is bright so that it can be interpreted visually by trained scientists The color of the spot is present on the chip, counted and a ratio of red-yellow and green-yellow is calculated which can be done by hand The color of the spot can't be converted None of the aboveWhich of the following is a correct statement about the primers used in the ALU insertion PCR reaction? Group of answer choices The primers are different for the + allele DNA sequence and the - allele DNA sequence The primers for both + and - alleles are the same and are on flanking regions to the insertion The primers are specific to the insertion DNA sequence The primers are made of proteins1. Definitions and brief statements 1. DNA Data Bank of Japan 2. MeSH Database 3. Please describe the 3 steps and temperature that are cycled during a PCR reaction. 4. What are global alignment and local alignment and their algorithms? ( 5. ProtScale
- Please submit a paragraph comparing two methods of DNA isolation (traditional with Qaigen DNA isolation kit method).How can someone distribute the DNA in the spots if they are producing a Vicrochip DNA microarray and it has enough space for all of the families that are known? From the following options which is the best choice. They will sort the DNA across the spots in an alphabetical arrangement according to the family so it's easier to read the data from the chip They will randomly and evenly distibute the DNA in the spots to reduce the chance of errors with distibution of the samples when they are applied They will organize the spots of DNA according to most rare viruses to most common viruses so it's easier to know if the sample is normal or abnormal They will place the DNA in spots according to how long the DNA pieces are so that the spots have a gradient of short hybridizing to long hybridizing pieces of DNA None of the aboveIt is common to see one or two diffuse (fuzzy) bands of RNA and/or primer dimer at the bottom of the gel after PCR amplification. RNA may be found at approximately the position of the smallest fragment of the PBR322/BstNI marker you used alongside your samples you loaded. RNA is the largest component of nucleic acid isolated from plant tissue, so some RNA may escape digestion by the RNase incorporated into the TE buffer in Part Il of the experiment. Primer dimer is an artifact of the PCR reaction that results from two primers overlapping one another and amplifying themselves. Where do you expect to see bands of Primer dimer and/or RNA, if present, on your gel? A. approximately 50bp and 121bp fragment of the marker B. below 121bp fragment of the marker C. between 121-383bp fragments of the marker D. none of these
- All of the following are performed during restriction fragment length polymorphism analysis. 1. splitting of double-stranded into single-stranded DNA 2. gel electrophoresis 3. autoradiography 4. immersion in radioactive probes 5. digestion of DNA with restriction endonucleases 6. use of a positive charge to transfer single-stranded DNA from a gel to a membrane. The correct sequence of these operations is whatRegarding STR markers used in forensic science. Tick all the correct statements: no correct statement the PCR primers used to amplify STRs are located in the repeat units PCR primers to amplify STRs are located on both sides of the repeat units the PCR primers used to amplify STRs are coupled to a fluorochrome which is essential for the detection of amplicons they are absent from the gonosomes the allelic frequencies of STR markers vary according to the ethnicity of the individuals genotyped they are present homogeneously throughout the nuclear genomeWhat is the sequence of the sample DNA submitted for sequencing given the gel electrophoresis profile from the Sanger sequencing method? ddATP ddCTP ddGTP ddTTP
