During RT-PCR, a poly-T primer and reverse transcriptase is used to turn all the mRNA to DNA. Why is a poly-T primer always used?     All mRNA contains a 5' poly-U tail     All mRNA contains a 3' poly-U tail     All mRNA contains a 5' poly-A tail     All mRNA contains a 3' poly-A tail

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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  1. During RT-PCR, a poly-T primer and reverse transcriptase is used to turn all the mRNA to DNA. Why is a poly-T primer always used?

       

    All mRNA contains a 5' poly-U tail

       

    All mRNA contains a 3' poly-U tail

       

    All mRNA contains a 5' poly-A tail

       

    All mRNA contains a 3' poly-A tail

     

Expert Solution
Step 1

RT-PCR, or reverse transcription polymerase chain reaction, is a widely used technique for the detection and quantification of RNA molecules. In RT-PCR, RNA molecules are first reverse transcribed into complementary DNA (cDNA) using a reverse transcriptase enzyme, and then the cDNA is amplified using the polymerase chain reaction (PCR).

 

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