During RT-PCR, a poly-T primer and reverse transcriptase is used to turn all the mRNA to DNA. Why is a poly-T primer always used?     All mRNA contains a 5' poly-U tail     All mRNA contains a 3' poly-U tail     All mRNA contains a 5' poly-A tail     All mRNA contains a 3' poly-A tail

Following PCR amplification of a disease gene, you perform sequence analysis using a primer 20 nuclear tides in length. partial results are depicted on the gel below which of the following are true?

The following is a sample primary RNA transcript. What must first occur before it can be translated? Exon 1 - Intron 1 - Intron 2 - Exon 2 - Intron 3 i) Exons need to be removed ii) Introns need to be removed iii) Poly A tail needs to be added to the 3' end iv) Poly A tail needs to be added to the 5' end v) 5' cap needs to be added O i, i, iv O i, iv, v ii, iii, v O ii, v

Please fill out the following table. Circle sense or template strands. Indicate 5’, 3’, amino and carboxy ends. Use wobble whenever possible

Based on the electron micrograph shown, which of the following statements is/are correct/true? Select all that apply. You may select mutiple options. DNA and mRNAs are marked by arrows. DNA mRNAs #3 Left Right 1 μm You cannot determine relative mRNA lengths from this image You cannot determine the direction of transcription from this image the longest mRNA is on the right side ✔Direction of transcription is left to right O Direction of transcription is right to left the 5' end of the mRNA marked #3 is at the bottom O the longest mRNA is on the left side the 5' end of the mRNA marked #3 is at the top You cannot determine the 5' or 3' mRNA ends from this image

When you are extracting DNA one of the first steps is to dissolve the cell membranes with ice cold ethanol.  True or False

You have purified a protein with that is comprised of 600 amino acids. How long (in nucleotides) is the mRNA transcript from start codon to stop codon? Group of answer choices 200 bases 600 bases 1200 bases 1800 bases

The following are the restriction enzyme sequences. The slash indicates the cut in the DNA sequence. EcoRI: 5' G/AATTC 3' Hind IlI: 5' AG/GATCC 3' Bam Hl: 5' G/GATCC 3' For DNA sequences 1,2 and 3 (below) Write the 3' to 5' DNA bottom strand • Write the mRNA sequence of top strand Write the mRNA sequence of bottom strand Write the Protein sequence of the Bottom strand • Which enzyme should be used to differentiate (detect the mutation) in sequence 2 1) cac agt aca tca gac atg gat cca agc cca tgt ata ccc cg aac 2) cac agt aca tca gac atg gtT cca agc cca tgt ata ccc ccg aac 3) cac agt aca tca gac atg gaC cca agc cca tgt ata ccc ccg aac Edit View Insert Format Tools Table 12pt v Paragraph

Which of the following features is common to both DNA replication and RNA transcription?     Both RNA and DNA polymerase form a sugar-phosphate bond between the 3' hydroxyl and the 5' phosphate.     Both RNA and DNA polymerase require oligonucleotide priming.     Both RNA and DNA polymerase incorporate deoxyribonucleotides into the growing sequence.     Both RNA and DNA polymerase initiate at promoter sequences.     Both RNA and DNA polymerase have extensive proofreading editing.

Which of the following is a section of MRNA produced from the DNA template below? 3' ATAGCTA 5' O 5'UAUCGAU 3' O 5'ATAGCTA 3' O 5'AUAGCUA 3 5'TATCGAT 3'