which of the following do researchers not need to use during vector cloning? a. a plasmid containing selectable marker genes such as beta galactosidase or ampicillin resistance genes. b. restriction enzymes. c. DNA polymerase  d. a growth medium with carefully selected ingredients that take advantage of selectable markers.  e. none of the above.

Q: A biotech lab technician is working with a mutated form of a particular bacterial gene and wants to…

A: Plasmids  are naturally occurring extra chromosomal double standard DNA molecules that replicate…

Q: A DNA microarray is a slide that is dotted witha. mRNAs from a sample of cells.b. fluorescently…

A: Bio-informatics is the branch of biology that uses computers and statistics to analyse and record…

Q: RELPs: A. are the same length for mutant and normal beta-globin alleles.  b. determine the sequence…

A: Approach to solving the question: Read through each statement carefully and determine which ones…

Q: Which of these statements is correct regarding the CRISPR/Cas9 system in eukaryotic cells? a.Single…

A: The game-changing CRISPR-Cas9 technology is changing the game of genome editing. The CRISPR-Cas9…

Q: PCR is used to: Select one: a. Randomly break large pieces of DNA or plasmids into smaller…

A: PCR ( polymerase chain reaction ) is used to amplify a specific segment of DNA . Amplification is…

Q: In 1996, a group of scientists from Scotland used a somatic cell of an adult sheep and fused it with…

A: Cloning

Q: The function of a restriction enzyme is to a. prevent the movement of DNA outside the nucleus b.…

A: Restriction enzymes:- these are the proteins and mostly isolated from prokaryotes. These enzymes…

Q: Choose the statement that best describes the outcome of transfecting cells with a plasmid that…

A: A plasmid is an extra chromosomal DNA present within a cell. It is physically separated from the…

Q: In biotechnology, an organism modified by gene splicing method is called Which of the following…

A:

Q: Match the activity below with the correct enzyme. (You won't use all the enzymes listed.) RNA acts…

A: DNA helicase is used during the DNA replication process where it binds to the double-stranded DNA…

Q: Which of the following cloning strategy will be best to clone multiple DNA fragment (~10 fragments)…

A: Gibson assembly is a type of DNA assembly method that allows for the joining of multiple DNA…

Q: A genetic map provides a. the sequence of the DNA in a genome. b. the relative position of genes…

A: The genome of an organism represents all the genetic information contained within the cells. This…

Q: When using gene therapy to treat an hereditary disease, the idea is to: A. Introduce the correct…

A: Genes are the functional segment of DNA which encode for proteins.

Q: function of a restriction endonuclease?

A: Recombinant DNA technology mainly aids in identification or investigation of DNA, recombination and…

Q: Which of the following is FALSE about current Sanger dideoxy DNA sequencing procedures?   a. Chain…

A: An oligonucleotide primer is annealed to a single-stranded DNA template and expanded by DNA…

Q: Good cloning vectors must have  a. an origin of replication. b. a site for inserting a gene.…

A: Vectors are the vehicles that carry the gene of interest from one organism to another. Vectors…

Q: In gene mapping using generalized transduction, bacterial genes that are cotransduced are a. far…

A: Transduction is a process used to map the bacterial genes. The transduction is of two types,…

Q: Which of the following approaches you can use to locate the relative positions of genes on…

A: Genes are located on chromosomes. These genes can not be targeted without using any specific gene…

Q: What is the enzymatic function of restriction enzymes? Group of answer choices a. to cut nucleic…

A: Restriction enzymes are involved in accomplishing various biotechnological processes. Mostly found…

Q: The CRISPR method is used in  a.DNA sequencing. b.DNA fingerprinting. c.genome editing. d.the…

A: CRISPER stands for clustered regularly interspaced short palindromic repeats. It is a group of DNA…

Q: During a PCR reaction, all the following events occur except: A. Hybridization B. Binding of a 3’OH…

A: PCR : it is polymerase chain reaction, it is a technique of molecular biology which is used to…

Q: Which of the following is NOT a true statement about CRISPR/Cas9? A. CRISPR/Cas9 is simpler to…

A: E. Delivery of CRISPR/Cas9 is not at all easy.

Q: A mutagen that is a base analog isa. ethyl methanesulfonate (EMS).b. 5-bromouracil.c. UV light.d.…

A: The DNA is a genetic material- a polynucleotide chain made up of the long chain of A, T, G, and C.…

Q: The restriction endonucleases used in recombinant DNA work:   a. are synthesized by bacteria   b.…

A: Specific sequences in the entering DNA are recognized by restriction endonucleases, which then break…

Q: When we discuss PCR and other similar techniques, the term Tm is often used. This refers to a.The…

A: PCR ( Polymerase Chain Reaction) is a technique in which numerous copies of genetic material (…

Q: Place the steps of sanger sequencing in order. A.  A fluorescent laser excites the fragments and…

A: DNA (the DNA that has to be sequenced) is used as the beginning point for DNA synthesis.…

Q: Define the following terms: a. ANT b. symporter c. phosphate translocase d. CK e. CK/PCr shuttle

A: Symporter: It is a integral membrane protein that involves in the transport of two different…

Q: Primers can sometimes bind and target the wrong gene, especially if the primers are allowed to bind…

A: It is difficult for one to to find out gene of interest out of a complete genome. However, a given…

Q: Which of the following cannot usually be inserted into a vector to form a recombinant DNA?

A: a vector is a DNA molecule that is used as a vehicle to transfer foreign genetic material into a…

Q: The ab initio approach finds genes by looking for a. common sequences found in most genes. b.…

A: Gene prediction is a computational biology process in which the encoding regions of a gene are…

Q: Which of the following methods use(s) a labeled nucleic acidprobe, such as a labeled fragment of…

A: DNA is the genetic material that carries genetic information in the form of coded nucleotide…

Q: The goal of most gene therapies is to insert a healthy copy of a gene into the genome.  Besides…

A: The genome of an organism is defined as the whole genetic information that is inherited from one…

Q: The polymerase chain reaction requires the following except A. primers complementary to the ends of…

A: Introduction: A specific DNA segment can be amplified in a laboratory setting using the Polymerase…

Q: Which of the following would be considered a transgenic organism? A. a human whose human…

A: Introduction :- An organism or cell is referred to as transgenic if its genome has been transformed…

Q: When bacteria are consumed by macrophages, the macrophages give off chemokines that recruit other…

A: Chemokines have many different activities, and in many cases, the microenvironment has a significant…

Q: amount

A:

Mitochondrial Mutations

A mitochondrion is a cellular organelle that serves as the site for cellular respiration and energy production (ATP or Adenosine Triphosphate). It is a double-membrane-bound organelle and is only present in eukaryotic cells.