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Nonenzymatic Reaction Rate (s-) Enzymatic Reaction Rate (s-) Enzyme Rate Enhancement Carbonic anhydrase 1.3 x 10-1 1x 106 7.7 x 106 Chorismate mutase 2.6 × 10-5 50 1.9 x 106 Triose phosphate isomerase 4.3 x 10-6 9- 4300 1.0 x 10° Carboxypeptidase A 3.0 x 10-9 578 1.9 x 10" AMP nucleosidase 1.0 x 10-1" 60 6.0 x 1012 Staphylococcal nuclease 1.7 × 10¬13 95 5.6 X 1014

Nonenzymatic Reaction Rate (s-) Enzymatic Reaction Rate (s-) Enzyme Rate Enhancement Carbonic anhydrase 1.3 x 10-1 1x 106 7.7 x 106 Chorismate mutase 2.6 × 10-5 50 1.9 x 106 Triose phosphate isomerase 4.3 x 10-6 9- 4300 1.0 x 10° Carboxypeptidase A 3.0 x 10-9 578 1.9 x 10" AMP nucleosidase 1.0 x 10-1" 60 6.0 x 1012 Staphylococcal nuclease 1.7 × 10¬13 95 5.6 X 1014

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Approximately how much does staphylococcal nuclease (Table) decrease the activation free energy ΔG‡ of its reaction (the hydrolysis of a phosphodiester bond) at 25°C?

Nonenzymatic Reaction Rate (s-) Enzymatic Reaction Rate (s-) Enzyme Rate Enhancement Carbonic anhydrase 1.3 x 10-1 1x 106 7.7 x 106 Chorismate mutase 2.6 × 10-5 50 1.9 x 106 Triose phosphate isomerase 4.3 x 10-6 9- 4300 1.0 x 10° Carboxypeptidase A 3.0 x 10-9 578 1.9 x 10" AMP nucleosidase 1.0 x 10-1" 60 6.0 x 1012 Staphylococcal nuclease 1.7 × 10¬13 95 5.6 X 1014
Transcribed Image Text:Nonenzymatic Reaction Rate (s-) Enzymatic Reaction Rate (s-) Enzyme Rate Enhancement Carbonic anhydrase 1.3 x 10-1 1x 106 7.7 x 106 Chorismate mutase 2.6 × 10-5 50 1.9 x 106 Triose phosphate isomerase 4.3 x 10-6 9- 4300 1.0 x 10° Carboxypeptidase A 3.0 x 10-9 578 1.9 x 10" AMP nucleosidase 1.0 x 10-1" 60 6.0 x 1012 Staphylococcal nuclease 1.7 × 10¬13 95 5.6 X 1014
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