Which E. coli enzyme is the main replicative polymerase in bacteria that synthesizes the genome?

Q: What is linker scanning mutagenesis?

A: Answer: Introduction: Old-style approaches, containing linker-scanning mutagenesis includes…

Q: What is the name of the DNA duplication process? What is the main enzyme that participates in it?

A: Every living cell divides to form multiple cells. During this division process, there is another…

Q: What are the five main steps in DNA cut-and-paste transposition?

A: The "cut and paste" method is used for saving conversion. The enzyme transposase acts as a pair of…

Q: What difficulties arise in the polymerase chain reaction if there is contamination of the DNA that…

A: Deoxyribonucleic acid or DNA is a nucleic acid that composed of two polynucleotide chain that is…

Q: What is a closed genome?

A: A pan-genome is also called as supragenome, which is the entire set of genes for all strains of…

Q: What is transformation in cloning?

A: Cloning is a process of taking the complete genetic information of an organism and recreating…

Q: If a mutagen causes bases to be removed from nucleotides withinDNA, what repair system would fix…

A: DNA repair is the process in which certain specialized enzymes within the cell known as DNA repair…

Q: Why must the gene be inserted into a vector for it to be cloned?

A: Cloning is the process by which a foreign DNA is inserted into the host cell for the purpose of…

Q: Which are the three types of RNA polymerases transcribe genes in Kukaryotes?

A: Transcription is the process by which the information in a strand of deoxyribonucleic acid (DNA) is…

Q: If a mutagen causes bases to be removed from nucleotides withinDNA, what repair system could fix…

A: By the process of mutation, bases are removed from the nucleotides.

Q: How is genome replicated in M13 bacteriophage?

A: M13 belong to the family of Ff phages. Ff phages are composed of a circular single stranded DNA…

Q: Why is cDNA used for cloning?

A: Cloning is the production of organisms, which are genetically identical to their parents. These…

Q: How can the CRISPR bacterial immune system be used to specifically and accurately edit genomes in…

A: CRISPR is a family of DNA sequences found in the genomes of prokaryotic organisms such as bacteria…

Q: Which molecule is responsible for producing the double-stranded breaks that initiate CRISPR genome…

A: The biochemical molecule that is built up with two polynucleotide chains is called Deoxyribonucleic…

Q: What enables RNA polymerase to start transcribing agene at the right place on the DNA in a bacterial…

A: The process that is characterized by the activity of RNA polymerase is called transcription and the…

Q: How was the first Copy Cat was cloned from a kitten?

A: Cloning is the practice of creating organisms, either spontaneously or intentionally, with similar…

Q: Why is ligase needed to make recombinant DNA? Whatwould be the immediate consequence in the cloning…

A: DNA replication is the process of copying the DNA of the cell during cell division. DNA ligase is an…

Q: What is the annotation of the genome?

A: A gene is the fundamental unit of heredity, physical and functional. Gens consist of DNA. Some genes…

Q: How can the CRISPR and pi-cluster loci change overtime?

A: CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeat. It is a unique…

Q: Why is the technique used to clone mammals called somatic cellnuclear transfer?

A: A somatic cell is a cell that takes part in the composition of the body of an organism and divides…

Q: How could DNA-mediated transformation be used to test chemicals for their mutagenic activity?

A: Deoxyribonucleic acid (DNA) is an inheritable entity of almost every living organism. It is…

Q: Why do bacteria make restriction endonucleases? What is it about the endonucleases that prevents…

A: Restriction enzymes cleave DNA at or close unique recognizing sequences known as restriction sites,…

Q: Why is bacteriophage Mu mutagenic, and whatfeatures are necessary for Mu to insert into DNA?

A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…

Q: How does PCR minimize the problems associated with degraded DNA? What factors can cause DNA to…

A: PCR is Polymerase chain reaction.It is a method widely used to rapidly make millions to billions of…

Q: How can the number of repeats of a microsatellite/STR increase?

A: A repetitive DNA tract wherein a particular motif of DNA is repeated 5 to 50 times is termed a…

Q: What is gene gun ?

A: The technique of tissue culture is used for genetic engineering. In this method tissue samples are…

Q: How many SNPs are in the human genome?

A: The human genome consists of about 3 million base pairs and 23 pairs of chromosomes. Out of these,…

Q: How does the use of restriction endonucleases of different specificities aid in the sequencing of…

A: Restriction endonucleases of different specificities cleave DNA at specific sites.

Q: Why is it more important for DNA to be replicated accurately than transcribed accurately?

A: In molecular biology, DNA stands for Deoxyribonucleic acid which is a type of nucleic acid. It is…

Q: Why does reversible chain termination sequencing yield short reads?

A: In genetics, sequencing is considered a methodology to determine the order of nucleotides(adenine,…

Q: Do DNA-polymerase enzymes also function as exonucleases?

A: DNA polymerase enzyme is primarily responsible for the synthesis of DNA by the attachment of various…

Q: What are the types of transposons? Please explain in detail how transposons contribute to genome…

A: Some mobile genomic components have been named as mobile genetic elements or transposable elements…

Q: What is CRISPR?

A: Introduction  Bacteria and viruses are tough competitor to each other, in order to overcome each…

Q: What is multiple cloning site ?

A: Introduction Molecular cloning is a set of molecular biology experimental methods for assembling…

Q: How Are RNA Genomes Replicated

A: Most of the genomes of viruses composed of RNA and replicated through use of RNA or as a template…

Q: What is the Pan genome  ?

A:

Q: What is CRISPR? What can it do?

A: Unlike eukaryotic cells in plants and animals, prokaryotic cells such as bacteria and archaea have…

Q: Who created the gene gun?

A: Any of the various substances that are generated by living organisms and cells are called…

Q: What is cloning vector?

A: Deoxyribonucleic acid (DNA) is a molecule made up of two polynucleotide chains, DNA carries all of…

Q: What is natural transformation in biology?

A: In biology transformation is the process of genetic alteration of a cell. In this process an…

Q: What is the significance of digesting the 16S rRNA product in 16s gene sequencing?

A: The 16S rRNA is a structural component of the 30S ribosomal subunit of  bacteria and archea.

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.