Why was there a greater A260 absorbance reading for your DNA sample that was incubated at higher temperatures?

You are given a tube containing 275 ng of purified PCR product (DNA) that is 1262 bp long. How many picomoles of PCR product are in the tube? The average molecular weight of a deoxynucleotide monophosphate is 328 g/mol.

Calculate the volume (in µL) of DNA template required to add 60.0 ng to a 40.0 µL PCR if the DNA template is at a concentration of 28.0 ng µL-1.

This individual's x-ray diffraction data helped to confirm that DNA was orientied in a helical structure with uniform diameter. O a. Rosalind Franklin b. James Watson O c. Francis Crick O d. Gregor Mendel e. Edwin Chargaff

In DNA isolation techniques, a washing step is always done prior to the final resuspension. What is the purpose of this step? In DNA isolation from blood samples, why does the vial for blood storage contain EDTA? In the preparation for DNA isolation in plants, the plant source is refrigerated and ground prior to extraction. Why is this so? Why are DNA pellets air-dried before resuspension in buffer?

Use the plasmid to answer the following questions a) Digest the plasmid with EcoRI only and then both EcoRI and BamHi together. Fill in the table to show the fragment sizes produced.

You isolate bacterial DNA from an unknown species, and use a PCR primer that amplifies DNA only from the bacterial genus Staphylococcus. After PCR and gel electrophoresis, you do not see any bands of DNA on your gel.What does this mean?

what is the purpose of calcium chloride in the transformation process?

You are trying to determine the base content for a number of samples in the lab (mouse DNA, bacterial DNA, single-stranded DNA virus and double-stranded RNA virus).  Unfortunately, you failed to use an alcohol-proof marker and during the ethanol precipitation of your samples the labels came off the tubes.  You finish the experiment anyway and try to use the data to determine the samples.  Can you determine the source of each sample based on your results?  Briefly explain how you come to your designations.   Sample % adenine % cytosine % guanine % thymine % uracil     A 18 32 32 18 0     B 26 18 32 24 0     C 31 19 19 0 31     D 10 40 40 10 0

In DNA extraction where ethanol was used why does the DNa float