When analyzing your results after performing a serial dilution, you obtain an average of 40 colonies on your 1 x 106 plate. How many cells were in the original sample?

If 0.1 ml of a 1 * 10−6 dilution plate contains 56 colonies, calculate thenumber of cells per ml of the original culture

A 0.00001 dilution is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 223 colonies of bacteria grow on the petri dish.  Assuming each colony came from a single bacterium, how many bacteria are in a single mL of the original culture? Express your answer to two decimal places using exponential notation. Since only 0.1 mL is put on the plate, this counts as an extra dilution!!! Any time less than 1 mL is transfered, a dilution is being performed. Any time more than 1 mL is transfered, a concentration is being performed.

How could you utilize fluorescence quenching in the different analytical application? Please answer shorty at your own words. Answer should be specific (4-5 lines)  .

Refer to the provided image drawn by a student trying to plan out their serial dilution protocol. The student diluted the original culture into bottle A and then diluted it further into B as shown. The student then proceeded with plating out 0.1ml of the culture from bottle B onto the plate (Note: plate A shows the 0.1ml that was plated, no further dilutions were done). If 13 colonies grew on plate A, help the student figure out how many CFU (colony forming units) were in the original culture? Select one: a.1,300 b.13,000 c.None of the Above d.130,000 e.1,000,000

How do you know if you have a ‘countable’ plate when doing a dilution series?   Two plates received 0.1 mL of the same dilution tube. The first has 293 colonies and the second has 158 colonies. Suggest a reasonable reason why this happened.

Draw a 4-inch circle. Draw out the streaking pattern used to isolate single colonies on a plate.

Ten grams of hamburger were added to 90 mL of sterile buffer. This was mixed well in a blender. One-tenth of amL of this slurry was added to 9.9 mL of sterile buffer. After thorough mixing, this suspension was further dilutedby successive 1/100 and 1/10 dilutions. One-tenth of a mL of this final solution was plated onto Plate Count agar.After incubation 145 colonies were present. How many colony-forming units were present in the total 10 gramsample of hamburger?

Describe the results for each test indicated below. Use full sentences and write your answers in proper paragraph form. Gram stain Positive or Negative (How did you know?) Cell arrangement Cell morpholog Phenol Red Fermentation Did fermentation occur in each sugar? What products were produced? (How could you tell?) Streak for Isolation Successful or unsuccessful? (How could you tell?) Pigmented or non-pigmented? describe any other characteristics of Colony Morphology

Performing a bacterial isolation by streaking, using sterile (aseptic) technique, consider the expected outcome on the streak plate after the following: Two organisms were in the original sample. Organism G was present at twice the abundance of Organism H (so 2/3 of the original sample). The streak plate was generated using 4 areas (quadrants)- A, B, C & D. Area A was inoculated with a loop containìng 3 mìllion organisms from the original culture, which was mixed very well and considered to be homogeneous. Areas B, C & D were streaked from the previous area with a sterilized loop, with each streak transferring 2% of the organisms from the previous area (assume the relative abundance of the two organisms was maintained). What is the expected outcome in Area on this streak plate? 2d