When analyzing your results after performing a serial dilution, you obtain an average of 40 colonies on your 1 x 106 plate. How many cells were in the original sample?

Q: You look at a slide you prepared and, despite the fact that you know you put organism onto the slide…

A: Gram-staining is a laboratory test that detects the presence of bacteria and also helps to…

Q: What would your final dilution be if you transferred 0.1 mL of a 10-6 dilution and added it to a…

A: The pour plate method is a technique used to count the number of colony-forming bacteria found in a…

Q: Meats are a poor source of folate because folate is destroyed by ______________________but you can…

A: Folate is naturalky present in wide variety of food like meat and raw vegitables .

Q: How could you utilize fluorescence quenching in the different analytical application? Please answer…

A: Fluorescence quenching is a significant method for estimating restricting partiality among ligands…

Q: You have a culture that has 5 X103 cfu/ml of bacteria in it. You perform a serial dilution by adding…

A: In microbiology and bacteriology, the technique of serial dilution is a fundamental method used to…

Q: Three grams of soil from the ground were added to 27 mL of sterile water and shaken vigorously.…

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Q: If you were working with an unlabeled simple stained smear, would you be able to identify the…

A: Staining is used to colour biological specimen such as cell to identify them. Simple staining…

Q: You aseptically transfer 1 ml of your optional liquid culture into 99 ml of sterile water. What is…

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Q: If you transfer 0.1 mL of culture into a 99mL of sterile water, then add 1mL of that to an agar…

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Q: A serial dilution of overnight E.coli culture was performed by pipetting 1ml of a bacterial culture…

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Q: Why is it important to use a sterilized loop between streaks when preparing a streak­plate?…

A: Streak plates are prepared to grow and isolate individual colonies of bacteria. Here, we will mark…

Q: step by step process of Methyl Red test using a schematic diagram

A: The Methyl Red Voges Proskauer protocol is used to identify and differentiate the microbes based on…

Q: You are tasked to isolate a certain species of bacteria from a mixed culture to further characterize…

A: Bacteria are small single-celled organisms. Bacteria are found almost everywhere on Earth and are…

Q: What are the advantages and disadvantages of viable cell count and the turbidimetric methods?

A: In turbidimetric determination of cell growth ,after inoculation of the bacterial culture the…

Q: You are incubating cells for 3 hours. The initial number of cells present in the sample were 102.…

A: Generation time is the time interval required for cells or a population to divide. Generation time…

Q: Which of the following methods of enumerating cells is the only one to detect live cells?

A: The cell culture is the process by which cells are grown under controlled conditions, generally…

Q: Five different strains of E. coli (1, 2, 3, 4 and 5) were streaked onto a series of tester plates.…

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Q: How do you know if you have a ‘countable’ plate when doing a dilution series?   Two plates…

A: The concentration of stock solution can be calculated by first diluting the stock into a suitable…

Q: Design a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL…

A: Serial Dilution -- Serial dilution are used to calculate the concentration of microorganism .It…

Q: To determine the number of cells in a pure culture of Klebsiella pneumoniae, you have performed a…

A: Colony-forming units per millilitre (cfu/ml) of Klebsiella pneumonia original culture can be found…

Q: you are given a mixed culture of s. aureus, E. coli and P. aeruginosa. how would you isolate each…

A: In the study of microorganisms, a mixed culture is one that contains more than one type of organism…

Q: What is the best streaking technique in a culture media? Explain

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Q: If the field diameter is 2.4 mm and there are 16 cells across the field of view when using the 40X…

A: Given, Field diameter - 2.4 mm Cells across the field of view - 16 cells Objective Lens - 40X The…

Q: You aseptically transfer 1ml of your original liquid culture into 999 ml of sterile water. What is…

A: The dilution factor may be expressed as the ratio of the concentration of stock solution to the…

Q: The actual length of a cell is 50µm. When projected on the big screen in the class, the same cell is…

A: Microscopy is a technique that is used to visualize objects that are not visible to the naked eyes.…

Q: What was the concentration of bacteria (CFU/mL) in the original suspensions used for dilutions and…

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Q: You perform the Methyl-Red (right) and Voges-Proskauer test (left). Interpret the results. Which…

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Q: The actual length of a cell is 50µm. When projected on the big screen in the class, the same cell is…

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Q: A sample is diluted by a factor of 10 five times. The 10^-3 dilution has 272 colonies on it.…

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Q: You have performed an experiment and your aim is to obtain pure colonies. However, you observe some…

A: Bacteria are prokaryotic organisms. They are microscopic single-celled organisms that can live in a…

Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.