You have performed an experiment and your aim is to obtain pure colonies. However, you observe some colony variations and you need to verify that this is not due to contamination, name and describe the staining method would you use to achieve this goal?

Q: The number of bacteria in a sample can also be quantified with other techniques, such as examination…

A: In microbiology laboratories the number of microorganisms present in a particular sample is…

Q: You look at a slide you prepared and, despite the fact that you know you put organism onto the slide…

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Q: You have a culture that has 5 X103 cfu/ml of bacteria in it. You perform a serial dilution by adding…

A: In microbiology and bacteriology, the technique of serial dilution is a fundamental method used to…

Q: Describe how the streak-plate method is used to obtain a pure culture and how the resulting culture…

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Q: A sample of water was enumerated using a counting chamber (haemocytometer) and 220 bacterial cells…

A: The viable plate count, or simply plate count, is a count of viable or live cells. It is based on…

Q: An acid-fast staining method was performed on a bacterial smear using the dyes carbolfuchsin and…

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Q: Step #1 Culture and perform a Gram-stain on the Super Bug You culture the Super Bug by swabbing the…

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Q: Please insert a photo of your cross-streaking for individual colonies and describe your results)…

A: The advantage of using solid media has been increased by describing the colony morphology, including…

Q: identify the organisms in  an unknown culture. You find that the culture contains two gramnegative…

A: A culture is a medium to grow bacteria and other microorganisms  It provides the bacteria with…

Q: ake a look at your streak plate from the use of a mix culture. Does your plate show the results that…

A: The streak plate method is a microbiological technique used for the isolation of pure strain ;…

Q: 45-year-old male had been working in his garden when he scratched the knuckle of his right arm…

A: There are many types of/species of bacteria. Through different techniques and lab procedures,…

Q: Which of the following serves as a counterstain for the gram-stain technique? It also happens to be…

A: Gram staining is a technique used to differentiate two groups of bacteria based on the different…

Q: For a laboratory exercise of basic techniques in microbiology: Gram stain and Microscopy. What are…

A: "Gram staining is important in microbiology because it allows scientists to quickly and easily…

Q: You have a culture of bacteria and you performed an Acid Fast staining. You see red rod-shaped…

A: Introduction -  Acid-fast staining is a differential stainingused to differentiate acid fast and non…

Q: You want to subculture your cells from T25 flask to 96-well plates. You first collected your cells…

A: A hemocytometer is an instrument that is commonly used to count the number of cells present in a…

Q: Describe one error in the staining procedure that would result in a Gram-positive bacterium…

A: Gram staining is a differential staining technique that differentiates between a gram-positive and a…

Q: Why would you use one single colony for both streaks on a plate and inoculate a culture for plasmid…

A: Introduction Plasmids are important for the transformation of bacteria. They are used as vectors and…

Q: Create paragraphs to differentiate among the following terms: positive stain, negative stain, simple…

A: An organic molecule with a benzene ring, a chromophore, and an auxochrome group is known as a…

Q: Refer to the provided image drawn by a student trying to plan out their serial dilution protocol.…

A: Colony forming unit (CFU)is a measure of viable bacterial cell. CFU/ml can be calculated using…

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Q: A patient sample will be analyzed. You are responsible to quantify the bacteria number. The dilution…

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Q: Colony of (bacteria): A cluster of cells (or clones) which arise from a single bacterium by asexual…

A:

Q: The statement, “In the laboratory, a sterile inoculating loop is moved across the agar surface in a…

A: The technique is streak plate technique. Streak plate method is a pure culture technique which helps…

Q: You and a partner conduct the acid-fast staining using the same two bacteria. You know that there…

A: Acid-fast staining is a differential staining technique that proves to be an important diagnostic…

Q: a.) Give the purpose of heat fixation. b.) What can be observed in wet mounts or hanging-drop slides…

A: The Purpose of fixation of a smear is as follows: 1) Tissue Killing- As this will help to prevent…

Q: Explain the principle of Gram staining. (You do not need to describe the procedure step by step).

A: The danish Bacteriologist Christian gram discovered this differential technique in order to…

Q: You make a smear of E. coli and then simple stain the slide with methylene blue. If you failed to…

A: The staining procedure is a multi-step process that involves the smear preparation followed by…

Q: Your first task in the lab is to learn how to culture these cells and begin expanding the culture…

A: Answer:  Yes, the cell culture media need to be changed more often than the other. The open culture…

Q: You are given a mix culture of S. aureus,E. coli and P. aeruginosa. Besides the streak plate method…

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Q: What is the purpose of fixing a smear? Mark all that apply: 1. To attach the bacteria to the slide…

A: The staining of microorganisms is very much important that helps us in identifying the morphology…

Q: When viable cell concentrations are too high to count on an agar plate, it is common to use larger…

A: The correct option is shown below.

Q: The main of objective of using 13-Line streaking method is to get:

A: Answer: 13-Line streaking method: It is the technique of obtaining pure bacterial culture by…

Q: Can you show the step by step process of Voges Proskauer Test using a schematic diagram?

A: Voges Proskauer(VP) Test is a biochemical method of detecting the presence of intestine-inhabiting…

Q: you are given a mixed culture of s. aureus, E. coli and P. aeruginosa. how would you isolate each…

A: In the study of microorganisms, a mixed culture is one that contains more than one type of organism…

Q: After performing the gram stain you observe your result as gram negative. To help confirm this…

A: Gram staining is a technique to identify bacterial stain gram-positive or gram-negative. It can be…

Q: You have a patient sample and have done a Gram Stain. When you look on the microscope, this is what…

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Q: Describe conventional techniques used to isolate single colonies and establish a pure culture

A: Answer: Introduction: Pure culture a culture contains growth of only single organism without other…

Q: What is the best streaking technique in a culture media? Explain

A: In microbiology, there are five I's that symbolise five different procedures used in the laboratory…