You have performed an experiment and your aim is to obtain pure colonies. However, you observe some colony variations and you need to verify that this is not due to contamination, name and describe the staining method would you use to achieve this goal?

What are the possible reasons why culture plates may have too many colonies despite performing serial dilutions and OD600 readings?

You and a partner conduct the acid-fast staining using the same two bacteria. You know that there will be an acid-fast bacteria present. Your slide shows acid-fast and non-acid fast bacteria. Your partners show both bacteria are non-acid fast. Your partner doesn’t understand why. What errors do you think occurred? Please state 2 and explain why these errors would cause this result.

Why is it necessary to cut thin sections of the tissue sample from a specimen using a microtome?

Your first task in the lab is to learn how to culture these cells and begin expanding the culture (increasing the # of cells) so that you may later conduct experiments on them.  You take two aliquots, each with an equal number of cells, and place them in two different culture vessels, one is a sealed airtight flask (culture 1) and the other is an open topped culture dish (culture 2). You provide both with the same growth media that contains their preferred fuel source, glucose.      Are the cells in cultures 1 and 2 deriving all their energy in the same way?  (Yes or No) Explain why or why not.  Be sure to name the specific processes by which each culture is deriving its energy.