How do you know if you have a ‘countable’ plate when doing a dilution series?   Two plates received 0.1 mL of the same dilution tube. The first has 293 colonies and the second has 158 colonies. Suggest a reasonable reason why this happened.

Q: What is the purpose of the RPR (RAPID PLASMA REAGIN )

A: Since you have asked multiple questions, we will solve the first question for you, If you want any…

Q: Why does counting 5 Boxes in each chamber of the hemacytometer gives you the number of cells in a ul…

A: Hemacytometer is a medical instrument used to measure the concentration of blood components in a…

Q: Why is the plate count method a determination of the number of viable cells?

A: The plate count method, also referred to as the viable count method or colony-forming unit (CFU)…

Q: How would you prepare a 1:500 dilution? You cannot use more than 10mL per tube.

A: In scientific research and experimentation, it is often necessary to dilute substances to achieve…

Q: 1. A plate with a final dilution factor of 107 produced 210 colonies. a) What was the original…

A: Dilution is the process of diluting or mixing two or more substances or compounds; it is also known…

Q: 10ul of the original sample was transferred to 90ul diluent. Then, the mixture was mixed with 100ul…

A: Hemocytometer is blood cell counting device. It is usally cposed of thick glass on which square…

Q: In a plate count, 1 mL of culture is spread on a plate and incubated for 24 hours. 250 colonies are…

A: Given,number of colonies in a 1mL of culture = 250. Also,given that this given solution is 100 times…

Q: How to count the number of colonies forming units per milliliter (CFUs per ml) of the sample?

A: A viable cell is one that has the ability to divide and form a colony. The colony-forming unit (CFU)…

Q: You have a culture that has 5 X103 cfu/ml of bacteria in it. You perform a serial dilution by adding…

A: In microbiology and bacteriology, the technique of serial dilution is a fundamental method used to…

Q: Describe how you would prepare a dilution series of a 1 x 107 CFU/mL culture to the 10-8 dilution…

A: Serial dilutions is a process of making step by step dilutions of a substance. It is formed by…

Q: What conclusion would you draw if the numbers of bacterial colonies in were the same on the control…

A: Bacteria are unicellular and prokaryotic organisms that live in wide variety of environments. It…

Q: You prepared 10-7, 10-8, and 10-9 dilutions of a bacterial suspension in sterile saline. Then you…

A: Introduction There are different culturing techniques such as agar plates, broth culture, stab…

Q: You want to create a 10-fold dilution . You have a saline tube with a volume of 450ul. How much of…

A: According to this theory, a diluted solution's volume will be 10 times greater than its stock…

Q: For an In vitro mimicking infection test:

A: Introduction: Cell culture is the process of growing cells in a controlled setting, usually outside…

Q: Below is a set of data for a serial dilution/plate count experiment.    Based on this data what is…

A: There are numerous methods/techniques for counting the number of bacteria in a particular sample. A…

Q: You want to subculture your cells from T25 flask to 96-well plates. You first collected your cells…

A: A hemocytometer is an instrument that is commonly used to count the number of cells present in a…

Q: Suppose your professor handed you a bottle of kombucha with 2.0 ml in it and told you to make a…

A:

Q: You counted 40 colonies on a plate in your dilution series. The plate was inoculated with 1.0ml from…

A: A microbial sample will have enormous amount of cells. To estimate this by calculation, the sample…

Q: You count 4 squares of a hemacytrometer and ontain 82,65,85 cells in each squy.what is the cell…

A: Hemocytometer is device used for the counting of blood cells in a sample. It has counting chambers…

Q: Remove the agar plate from its lid with the left hand. While holding the plate, inoculate the agar…

A: Introduction -- Microbes are around and every where in environment. Sterile agar plates which…

Q: n the Methyl Red-Voges Proskauer test, the samples were not transferred into sterile tubes for…

A: These tests are done to check which fermentation pathway is taken by pathogen to ferment glucose.…

Q: A patient sample will be analyzed. You are responsible to quantify the bacteria number. The dilution…

A: A sample of your blood, urine, skin, or any part of your body will be taken during a bacteria…

Q: e the process to make a 4-phase streak plate beginning with your first streak (i.e you have…

A: The sample/inoculum is usually pored by streak with the diluted through streaking it across the…

Q: A tampered sack of powdered milk was analyzed by getting 50 grams of composited sample and diluting…

A: Dilution : We know that the solute of the concentration will be decreasing in solution . There is a…

Q: Calculate the concentration of the overnight stock culture in CFU/mL

A: Colony-Forming Units (CFUs) refer to the number of individual colonies of any microorganism that…

Q: How do eosin-methylene blue (EMB) agar plates work? What organism(s) are they designed to detect?…

A: Several tests are performed in the laboratory in order to detect several bacterial agents as well as…

Q: in most probably number (MPN) testing what media is used for confirming test and what does a…

A: The Most Probable Number (MPN) is a method for estimating the concentration of viable microorganisms…

Q: Indicate whether you think that the number of cfu/ ml calculated would be too high or too low if you…

A: Cfu refers to the colony forming unit which is formed through continuous replication of a single…

Q: Describe three problems associated with using the standard plate count method for determining the…

A: Typically, an accurate count of bacterial colonies is required for biological processes. Microbial…

Q: Mary wanted to know the amount of microbes in her garden. To do this, she took a 10g soil sample,…

A: Serial dilution is a process done to reduce the microbial count in the sample. This is specifically…

Q: what would you conclude if an inoculated plate had colonies between the streak lines

A: Streaking is a method of isolating a pure strain from single species of  microorganism . in this the…

Q: Why must a discard tube should be collected first before an anticoagulated tube when using a…

A: Venipuncture is the most common method of blood collection. Blood is normally drawn from the medial…

Q: zone size

A: Antibiotics:These are the medications used to treat the bacterial infections. The appropriate…

Q: You plated 0.1 ml of a sample of kombucha diluted by a factor of 10^-3. It produced 43 colonies.…

A: The serial dilution process is used to calculate the number of microorganisms (bacteria) within a…

Q: Can you show the step by step process of Voges Proskauer Test using a schematic diagram?

A: Voges Proskauer(VP) Test is a biochemical method of detecting the presence of intestine-inhabiting…

Q: A culture of S. cerevisea has an overnight OD of 2.3 (1.0 OD is approx 1.0x107 cells/ml) You will be…

A: Dilution is the process of reducing the concentration of a solute in a solution. This is typically…

Q: A serial dilution was performed for a viable count of bacteria. The original bacterial cuture…

A: Bacteria are unicellular organisms, they grow as colonies. The colonies can be visualized with the…

Q: You wish to determine viable counts on a culture of Bacillus subtilis. You begin by pipetting 1 ml…

A: Answer given in step 2 and onwards. According to our guideline i will answer only one question.

Q: How would you produce a 10^-1 dilution if a 3 mL bacterial sample using the entire 3 mL volume?…

A: Dilution factor refers to the ratio of the volume of the initial (concentrated) solution to the…

Q: How do we reconcile the number of colonies in the plates to the actual number of bacteria in the…

A: Microbes may enter the body in a variety of ways and cause infection everywhere, but antigen and…

Q: You have 1 ml of kombucha. You remove 0.1 ml of this and mix it with 9.9 ml of fresh tea. You mix…

A: The following formula is used for finding the calculation of concentration of bacteria: CFU/ml =…

Q: You were instructed to add 1.0ml out of 4.0ml of an undiluted sample to 99ml of sterile diligent you…

A: Dilution factor is the ratio of volume of the initial (concentrated) solution to the volume of final…

Q: What are the possible reasons why culture plates may have too many colonies despite performing…

A: There are several possible reasons why culture plates may have too many colonies despite performing…

Health Assessment