What was the concentration of bacteria (CFU/mL) in the original suspensions used for dilutions and plating below:  Dilution Amount plated # of colonies counted 10-4 1.0 mL 271 10-5 0.1 mL 36 10-6 1.0 mL 9

Q: Why is skin contamination such a big problem in blood culture test?

A: Contamination can arise during the preparation of the venipuncture or catheter insertion site, the…

Q: You have performed a serial dilution of an unknown sample and counted 73 CFU on a countable plate…

A: Serial dilution is method of diluting a bacterial culture in succession. The process reduces the…

Q: One ml of a water sample was placed in a Petri dish with melted agar. After incubation, 129 colonies…

A: A colony-forming unit, or CFU, is a measurement of the concentration of microorganisms in a test…

Q: A culture is incubated for 10 hours. 1 hours after inoculation it reached the exponential growth…

A: This question involves the calculation of the growth constant (k) and generation time (g(t)) for a…

Q: 1 ml from main culture 1ml 99999 5 ml LB 1 ml 5 ml LB 5ml LB 1ml 5 ml LB 1 ml 5 ml LB You took 250…

A: To calculate the number of cfu/ml bacteria in the sample, we need to use the dilution factor and the…

Q: transferred to the plate. After 24 hours there were 60 colonies on the plate. What is the dilution…

A: b. The dilution factor is 1000. The patient sample contains 600,000 cells/ml

Q: You make a 1:100 dilution of your culture and plated 0.1 ml of this sample on a Nutrient agar plate.…

A: The amount of organisms present in the original culture is called as colony forming unit per…

Q: Which of the following plates (dilution factor) would you count/use to determine the original…

A: The colony-forming unit is the estimated number of bacterial cells in the culture that are viable…

Q: which bacteria is Coccobacillary,gram negative, non motile and negative oxidase test?

A: Bacteria are microscopic organisms which belong to prokaryote because these are unicellular…

Q: Use the information in the tables below to determine the most probable number of bacteria per 100 mL…

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Q: You are working in a medical lab and receive a urine sample from a patient that is displaying…

A:

Q: A serial dilution of a sample has an unknown concentration of microorganisms. A countable plate has…

A:

Q: You prepared 10-7, 10-8, and 10-9 dilutions of a bacterial suspension in sterile saline. Then you…

A: Introduction There are different culturing techniques such as agar plates, broth culture, stab…

Q: this lab was carried out the results  are in the picture below plz provide a aim for this lab abd…

A: Bacterial inhibition refers to the ability of a substance or agent to prevent the growth or…

Q: Negative Control Unknown Isolate

A: Blood agar comes as a basal media and can be used as a general growth medium.  Blood agar is an…

Q: If each agar plate contained 20 ml of LB agar and had an ampicillin concentration of 150 ug/ml, what…

A: Given data:  Each plate contains-  LB agar=20ml Ampicillin= 150 micrograms per ml.  Amount of…

Q: RESULTS Results of the plate counts for the samples you worked with. Number of Colonies for Each…

A: Cfu/ml= (no. Of colonies plated× dilution factor)/volume plated.…

Q: yield

A: The Dische's test is a chemical test that is utilized to detect the presence of DNA in a solution.It…

Q: What do to do when the disstillation did not make the sample completely pure? What could have went…

A: Distillation is a process that is based upon the boiling points of various liquids present in the…

Q: Which of the following is the a-anomer? H но H Но H2C H ОН H2C- нн ОН со 4 1 2 1 Submit 3 -OH Н ОН…

A: Alpha anomer

Q: Why is it best to use sterile distilled water in the preparation of microbial suspension and dry…

A: Smear preparation is the first step carried out during the staining procedure.

Q: Provided with the following data, compute the corresponding CFU/ml of the original culture. ssume…

A: Ideally, plates with colony 25-250 colonies are used. As above 250 colonies are too numerous to…

Q: A patient sample will be analyzed. You are responsible to quantify the bacteria number. The dilution…

A: A sample of your blood, urine, skin, or any part of your body will be taken during a bacteria…

Q: The unknown observations are shown as a mixed stain, gram positive, and gram negative. The next…

A: There are two types of bacteria present i.e., gram positive bacteria and the gram negative bacteria…

Q: The students of a Microbiology class were tasked to transfer or subculture a pure culture of…

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Q: A stool specimen from a 20-year-old man was sent to the Oregon State Public Health Laboratory for…

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Q: Joseph, being a know-it-all, told Mary that her method would not include all microbes. To…

A: Joseph and Mary had a disagreement about the effectiveness of Mary's method for counting microbes in…

Q: Calculate the concentration of the overnight stock culture in CFU/mL

A: Colony-Forming Units (CFUs) refer to the number of individual colonies of any microorganism that…

Q: You perform a serial dilution of a water sample and the 10-7 tube when 0.1 ml was plated onto the…

A: Estimation of the number of viable cells is important in microbiology protocols. It is estimated…

Q: colony-forming units

A: Colony Forming Unit:It is designated as CFU.It can defined as the unit of measurement used to…

Q: What is the difference between a Hemin test and a Benzidine test?

A: Hemoglobin is a blood protein that is responsible for oxygen transport in the tissues. Hemoglobin…

Q: Procedure Mix Take ml Top up to 50ml. Add 50 ul of Sml mixture Add fresh LB ampicillin to the…

A: Absorbance: The amount of light absorbed by a solution is measured by absorbance (A), also known as…

Q: What is the importance of the Nitrate test? What information can we gather from this test? Please…

A: Nitrate broth used in the nitrate test contains nutrients and potassium nitrate as a source of…

Q: On the basis of the appearance of this place, what are you testing for? When you added a reagent to…

A: Catalase is an enzyme that reacts with hydrogen peroxide to form water and oxygen. Catalase enzyme…

Q: You are testing a sample of water from a swimming pool. You count a plate made from a 1 mL transfer…

A: Dilution is a process by which solution (water or diluents) are added to the solvent (main solution)…

Q: You are given a mix culture of S. aureus,E. coli and P. aeruginosa. Besides the streak plate method…

A: Microbiological research necessitates the use of pure cultures. Spreading bacteria on the surface of…

Q: Which of the following plates (dilution factor) would you count/use to determine the original…

A: Dilution factor is a measure of the decrease in concentration of a solution that occurs in a…

Q: Mary wanted to know the amount of microbes in her garden. To do this, she took a 10g soil sample,…

A: Serial dilution is a process done to reduce the microbial count in the sample. This is specifically…

Q: Αγελα

A: based on above images the name of pathogen are given on visualize analysis.  following are the name…

Q: 50 g of food is homogenized in 450 mL of water. From this, 1 mL is added to 29 mL of water, and then…

A: Whenever a microrganism is plated for analytical purposes, it population is reported in terms of…

Q: At the station there is a 2 mL microcentrifuge tube containing sheep blood and 3 tubes containing…

A: Homolysis or hemolytic cleavage is the cleavage of chemical bond usually in the presence of light or…

Q: You plated 0.1 ml of a sample of kombucha diluted by a factor of 10^-3. It produced 43 colonies.…

A: The serial dilution process is used to calculate the number of microorganisms (bacteria) within a…

Q: How can you make a final dilution of 10-10  using 5 dilution tubes? Outline your steps.

A: Dilution is generally done to decrease the concentration of a solute in a solvent.

Q: How many bacteria would be in 1ml of the sample if you counted 134 colonies on the 1:1,000 dilution?

A:

Q: Why is skin contamination such a big problem in blood culture test?

A: Contamination can occur during venipuncture or catheter insertion site preparation, collection set…

Q: Why are only few drops of CuSO4 solution added during the biuret test?

A: Biuret test also known as Piotrowski's test, is a chemical test used for detecting the presence of…