What volume of 0.1M NaOH (reagent) needs to be added to increase your 7.5 pH (buffer) by 0.5 pH units. The goal is to further purify MOPs (see image).  Protonated form = 6.6 x 10.3^-3 mol/L Deprotonated form = 1.32 x 10^-2 mol/L Show calculations.

80mL of a 0.3M solution of hexapeptide Leu-His-Cys-Glu-Asn-Arg is adjusted to pH=pI. The solution is then titrated with 0.2M HCL to a final pH of 2.1. Sketch the titration curve, labelling the pH and volume axes. Indicate the volume of HCL needed to reach relevant pKa value and equivalence point(s)z Relevant pKa values are: 2.1, 4.3, 6.0, 8.3, 9.8, and 12.5.

Describe the preparation of 2.00 L of 0.100 M glycine buffer, pH 9.0, from glycine and 1.00 M NAOH. What mass of glycine is required, and what volume of 1.00 NaOH is required? The appropriate pK, of glycine is 9.6.

Calculate the pH of a buffer that contains 0.75 M acetic acid and 0.35 M acetate ion in 1 L solution. What will the pH of the buffer be upon the addition of 100.0 mL of 1.0 M NaOH? (Ka of acetic acid is 1.75 x 10-5 M)

You have been provided with stock solutions of: stock A:     0.06 M sodium pyrophosphate buffer pH 8.5 stock B:     3 M ethanol stock C:     0.015 M NAD+ stock D:     milli Q water Determine the volume you will need of each solution to prepare a buffer of with a final volume of 60 mL containing 10 mM sodium pyrophosphate pH 8.5, 100 mM ethanol, 1 mM NAD+. i.e. volume of stock A = _________mL volume of stock B = _________mL volume of stock C = _________mL volume of stock D = _________mL Show your calculations to arrive at your answers.

Calculate the volume of TAE buffer that you will need to prepare a 100ml solution of 1X strength if you only have a 50X concentrated solution available.

Acetic acid is the principal ingredient in vinegar as shown; that's why it tastes sour. At equilibrium, a solution contains [CH3CO2H] = 0.0787 M and [H3 O+] = [CH3 CO2−] = 0.00118 M. What is the value of Ka for acetic acid?

Identify the acid and conjugate base in each reaction. Calculate the pKa for each acid. List them in order from the strongest to weakest acid. The acid-ionization constants, Ka, at 25°C are listed for each. a. HC2H3O2 + H2O ↔ H3O+ + C2H3O2-acetic acid, KA = 1.7 x 10-5 b. HC7H5O2 + H2O H₂O+ + C7H5O2-benzoic acid, KA= 6.3 x 10-5 c. HC6H4NO2 + H2O ↔ H3O++ C6H4NO2-nicotinic acid, KA = 1.4 x 10-5

Using your stock solution of 2 M glucose, you need to prepare the following series of glucose solutions for use in an experiment 0.8; 1.5, 1.75 and 2 M. Complete the table to show how you would dilute the sucrose to make 100ml of each of the solutions above.

Calculate the appropriate volume (in µL) of 9X loading buffer that should be added to 46.0 µL of a sample prior to loading the sample on an agarose gel.