Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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B. You will need approximately 1 ml of the 100 µg/ml bromophenol blue (which is your most
concentrated standard) to generate your standard curve. Calculate how much bromophenol
blue stock solution and how much water you will combine to accomplish this. Make 1 ml of
this solution using a 100-1000 μl micropipetter. Use the equation C₁V1=C2V2
μl of 1 mg/ml stock +
μl of water = 1 ml of 100 µg/ml bromophenol blue
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Transcribed Image Text:B. You will need approximately 1 ml of the 100 µg/ml bromophenol blue (which is your most concentrated standard) to generate your standard curve. Calculate how much bromophenol blue stock solution and how much water you will combine to accomplish this. Make 1 ml of this solution using a 100-1000 μl micropipetter. Use the equation C₁V1=C2V2 μl of 1 mg/ml stock + μl of water = 1 ml of 100 µg/ml bromophenol blue
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