What is the difference between a map-based approach to sequencing a whole genome and a whole-genome shotgun approach?

Q: What is a database? What types of information are stored within adatabase? Where does the…

A: The complete set of chromosomes present in the organism is known as the genome. Bioinformatics…

Q: What is one major way in which next generation sequencing differs from dideoxy sequencing (Sanger)?

A: The technique of sequencing DNA involves identifying the nucleic acid sequence, or the arrangement…

Q: What is a simple way of making a knockout mouse in terms of DNA recombinant technology?

A: A knockout mouse, or knock-out mouse, is a genetically modified mouse in which researchers have…

Q: Which is a limitation of using massively parallel sequencing ?

A: It is a term used to describe the method of high-throughput DNA sequencing to determine the entire…

Q: What are the emerging techniques and challenges in utilizing multi-omics data for comprehensive…

A: The rapid advancements in sequencing technologies have allowed us to generate a wealth of data on…

Q: Differentiate between Sanger sequencing and next generation sequencing?

A: The process of determining the nucleic acid sequence is known as DNA sequencing.

Q: Explain Two strategies for assembling whole genome sequences.

A: All the genetic information of an organism is known as its genome. Genome assembly is defined as the…

Q: How can I develop a vector to express a bacterial gene (2,500 bp in size) in E. coli. How do I know…

A: Hi! As you have posted multiple questions, I will be answering the first two questions for you. If…

Q: There are four possible observations that suggest an error occurred in a PCR reaction: a) Sequence…

A: PCR reaction is a method used to amplify a small sample into millions of small ones which help the…

Q: Describe step 1: hybridization of template and primer in automated sanger sequencing

A: Introduction  Sanger sequencing is also known as the “chain termination method”. It is a method for…

Q: What is hierarchical sequencing?

A: Basically there are two approaches for the sequencing of large complex genome. They are hierarchical…

Q: What provides a convenient bridge between the low resolution of a karyotype and the ultra-high…

A: Introduction  Cytology refers to the study of cell such as cell morphology, physiology etc. As we…

Q: list and explain two strategic between the shotgun sequences/ assemble strategy for whole genome…

A: Genomic Sequencing is a process where whole genome of an organism is sequenced. With the help of…

Q: What is synthetic or artificial genome ?

A: A synthetic genome is an artificially built genome that is formed by processes involving genetic…

Q: How is recombinant DNA inserted into a genome using CRISPRediting?

A: The modern technology of genetics can be utilized to increase the genomes of various living…

Q: What is the difference between using genome build hg18 and hg19 in Genome-wide SNP arrays?

A: SNP (Single nucleotide protein) array is a type of DNA (deoxyribonucleic acid) microarray which is…

Q: What is Human Genome Project? What are its goals and benefits? What are the ethical, legal, and…

A: The genome is made up of genes and genes are made up of DNA. DNA is present inside the chromosomes…

Q: What is using somatic nuclei of transgenic adults to generate other animals with identical genomes?

A: Genomics refers to structure, function, evolution, mapping, and editing of genomes. Somatic cells…

Q: What does whole exome sequencing test for?

A: Exomes are part of the genome, which contains only the coding portions of the genes that is the…

Q: How to use a microarray for transcriptome analysis ?

A: Transcriptome is the complete set of the mRNA molecules expressed in an individual . It is termed as…

Q: process of overlap assembly in whole genome sequencing

A: Genome sequencing Genome sequencing or gene sequencing is a way of arranging nucleotides the n the…

Q: What are some possible research questions and practical applications that could be addressed by…

A: The engineering and manipulation of an organism’s genetic material on the scale of entire genome…

Q: What

A: INTRODUCTION:- Shotgun sequencing involves randomly breaking up DNA sequences  into lots of small…

Q: What is the motive behind Genome Project 10K?

A: The advent of low-cost, high throughput sequencing has ushered in a new age of genome science and…

Q: What is shotgun cloning or shotgun sequencing ?

A: Cloning is the process of creating an identical copy of something. In biology, it refers to the…

Q: How many male/female sheep are needed to clone one? Cancloning be done by using adult cells?

A: One male and one female sheep is required to clone one because we need one male gamete and one…

Q: When is low diversity especially problematic in sequencing?

A: In sequencing micro RNA any method which requires use of in house barcodes may results in giving low…

Q: What is maxam and gilbert method of sequencing

A: DNA (deoxyribonucleic acid) sequencing is a procedure to determine the order of nucleotides…

Q: What is the one main advantage of the whole-genome shotgun strategy over the hierarchical approach?

A: Genome sequencing is a vital process that helps in biological and medical analysis.

Q: How is genomic DNA prepared for shotgun sequencing?

A: Genomic DNA prepared for shotgun sequencing as follows :  1. ligating random genomic DNA fragments…

Q: What is CRISPR? Give two applications for this technology.

A: CRISPR, which stands for Clustered Regularly Interspaced Short Palindromic Repeats, is a family of…

Q: Describe the three basic goals of the Human Genome Project. What are at least three things we have…

A: The HUMAN GENOME PROJECT was a 13-year plant which was proposed by Frank Collins and Roderick. Thee…

Q: What is Sanger sequencing? Why do we use ddNTP? How to read a DNA sequence gel? c. What is a cDNA…

A: Your question contains multiple sub-parts, and as per our policy, we only answer the first three…

Q: CRISPR/Cas9 mediated genome editing has provided an unparalleled means of manipulating the genome.…

A: CRISPR/Cas9 (Clustered regularly interspaced short palindromic repeats/CRISPR-associated genes) is a…

Q: What is the major difference between the strategies of map based sequencing and shotgun sequencing?

A: DNA sequencing is a process in which sequence(order) of nucleotides in DNA is determined. It…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.