What is an STS? How are STSs generated experimentally? What are the uses of STSs? Explain how a microsatellite can be a polymorphic STS.

does human contain supercoiling DNA？Why supercoiling is important and what is it？

HOw many times can DNA segments be transcribe? Also, can mRNA molecules be reused?

Discuss the processes of conjugation, transformation (Griffith’s experiment), andtransduction. What is transposon gene?

What is the main or primary use of positional cloning?

How many male/female sheep are needed to clone one? Cancloning be done by using adult cells?

What is a single-nucleotide polymorphism (SNP)? How are SNPs used in genomic studies?

What is inverse PCR? How are we going to use inverse PCR to help figure out the molecular location of the EP element on Drosophila melanogaster flies?

When performing QPCR to study differential gene expression, what is the role of SYBR green? What is the most correct answer? SYBRgreen intercalates proportionally and efficiently with double stranded DNA and can therefore be used to quantify it   SYBRgreen intercalates proportionally and efficiently with nucleic acids and can therefore be used to quantify them.   SYBRgreen intercalates more efficiently and proportionately with single stranded DNA and can therefore be used to quantify cDNA (a direct product of mRNA).   SYBRgreen intercalates proportionally efficiently with messenger RNA and can therefore be used to quantify it.

According to the double-strand break model, does gene conversionnecessarily involve DNA mismatch repair? Explain.