What is hierarchical sequencing?

Q: What is linker scanning mutagenesis?

A: Answer: Introduction: Old-style approaches, containing linker-scanning mutagenesis includes…

Q: What is metagenome sequencing?

A: Metagenomics is the study of microbial communities in their environment by using modern genomic…

Q: Why is genome database and annotation relevant to Genome sequencing?   Are there any examples?

A: Genome: the set of genes. Genome database: it is the collection of all information on the human…

Q: What are genetic mapping studies?

A: Gene mapping is used to map the relative positions of genes on a chromosome using a cross over…

Q: What is positional cloning?

A: Gene cloning is the process in which any gene of interest is amplified, that is, several copies of…

Q: Which is a limitation of using massively parallel sequencing ?

A: It is a term used to describe the method of high-throughput DNA sequencing to determine the entire…

Q: Why is Sanger sequencing sometimes referred to as "dye-terminator" sequencing?

A: Frederick Sanger and his colleagues created Sanger Sequencing, often known as 'chain termination…

Q: What is exome sequencing used for?

A: DNA sequencing:   It is a method to find a sequence of nucleotides by using the DNA sequencing…

Q: Differentiate between Sanger sequencing and next generation sequencing?

A: The process of determining the nucleic acid sequence is known as DNA sequencing.

Q: What is the principle of next generation sequencing?

A: The process of determining the nucleic acid sequence is known as DNA sequencing.

Q: What is Sanger sequencing used for?

A: DNA sequencing is defined as a process in which DNA’s nucleotide sequence is determined. The main…

Q: Who proposed shotgun sequencing?

A: The method used for the sequencing of random DNA strands is genetics, shotgun sequencing.  This is…

Q: What are gene microarrays? How are microarrays used?

A: The process of forming a single double-stranded nucleic acid molecule by combining two…

Q: What provides a convenient bridge between the low resolution of a karyotype and the ultra-high…

A: Introduction  Cytology refers to the study of cell such as cell morphology, physiology etc. As we…

Q: What is synthetic or artificial genome ?

A: A synthetic genome is an artificially built genome that is formed by processes involving genetic…

Q: What is genomics, and how does structural genomics differ from functional genomics? What is…

A: Gene is a functional unit of heredity. A gene is a sequence of nucleotides in genome that codes for…

Q: What is involved in DNA sequencing?

A: DNA stands for deoxyribonucleic acid. It is the genetic material of the organisms that transfer from…

Q: How is recombinant DNA inserted into a genome using CRISPRediting?

A: The modern technology of genetics can be utilized to increase the genomes of various living…

Q: What is the purpose of shotgun sequencing?

A: Deoxyribonucleic acid (DNA) sequencing is the process of determining the nucleic acid sequence based…

Q: What is exome sequencing ? Why it is important ?

A: The exome has historically been described as the sequence in the genome that includes all exons of…

Q: What are the Strategies for genome sequencing?

A: The genetic material in the cells is present in the form of DNA. It contains the coded form of…

Q: What is the annotation of the genome?

A: A gene is the fundamental unit of heredity, physical and functional. Gens consist of DNA. Some genes…

Q: What is gene tagging?

A: Introduction Gene functions as the key to control almost all the physiochemical, biochemical…

Q: What does whole exome sequencing test for?

A: Exomes are part of the genome, which contains only the coding portions of the genes that is the…

Q: What is RNA sequencing ? Why it is important ?

A: The biochemical material that is carried from the preceding generation to the succeeding generation…

Q: what are similarities and differences between chain-termination and reversible terminator…

A: Gene sequencing  aids in the development of gene therapy, a form of treatment that replaces faulty…

Q: What are the two methods used for sequencing in Human genome project.

A: Answer- Human genome project was the mega project that aimed for sequencing of whole human genome.

Q: What is a open genome?

A: Open genomes main aim is to provide enhance basic research , improve public health by combining the…

Q: What

A: INTRODUCTION:- Shotgun sequencing involves randomly breaking up DNA sequences  into lots of small…

Q: What is a read (Sanger sequencing)?

A: DNA sequencing is the process of determining the sequence of nucleotides (adenine, guanine,…

Q: How are different variable region sequences generated?

A: Ans: The variable regions of the antibody have two chains heavy and light chains. They form a part…

Q: What is the basic principle of positional cloning?

A:

Q: What is comparative DNA?

A: I think the question was supposed to be about comparative genomics rather than DNA. I am answering…

Q: What is shotgun cloning or shotgun sequencing ?

A: Cloning is the process of creating an identical copy of something. In biology, it refers to the…

Q: When is low diversity especially problematic in sequencing?

A: In sequencing micro RNA any method which requires use of in house barcodes may results in giving low…

Q: What is the principle of Sanger sequencing?

A: DNA sequencing is the process of determining the sequence of nucleotides (adenine, guanine,…

Q: How is genomic DNA prepared for shotgun sequencing?

A: Genomic DNA prepared for shotgun sequencing as follows :  1. ligating random genomic DNA fragments…

Q: What is Genomics? Why genomic studies are important?

A: The genetic material present in most of the organisms present on earth is DNA. DNA holds the…

Q: How do you determine genome size?

A: The genome size refers to the amount of DNA in a haploid genome. It is expressed either in terms of…

Q: What is CRISPR?

A: Introduction  Bacteria and viruses are tough competitor to each other, in order to overcome each…

Q: What is the difference between a map-based approach to sequencing a whole genome and a whole-genome…

A: Deoxyribonucleic acid (DNA) is a double stranded helical genetic material containing thousands of…

Q: What is the major difference between the strategies of map based sequencing and shotgun sequencing?

A: DNA sequencing is a process in which sequence(order) of nucleotides in DNA is determined. It…

Q: What are steps of sequencing?

A: Introduction: Considering that it is DNA sequencing.  DNA is the genetic material of a living cell.…

Q: What is CRISPR? What can it do?

A: Unlike eukaryotic cells in plants and animals, prokaryotic cells such as bacteria and archaea have…

Q: Which are the limitations of using massively parallel sequencing to sequence a genome?

A: Massive parallel sequencing, also known as next generation sequencing or second generation…

Q: What is cloning vector?

A: Deoxyribonucleic acid (DNA) is a molecule made up of two polynucleotide chains, DNA carries all of…

Q: What is functional genomics? How does it differ from comparativegenomics?

A: The study of whole genomes of organisms is termed as genomics and it uses a combination of…

Q: What is single molecule sequencing in real time (SMRT) ?

A: DNA sequencing is a technique for determining the order of the four nucleotide bases found in DNA:…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.