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- t/Root/Pages/TestTaker/MonitorLaunch.aspx?id%3DE9B80AE3-962E-4628-B79D-A3F06F1CAB72 MonitorLaunchaspx?id%3DE9B80AE3-962E-4628-879D-A3F0EFICABT2-Google Chrone uninoculated tube The medium shown here was inoculated with a bacterium then incubated for 48 hours; an uninoculated control tube is also present for comparison. This particular bacterium gave a for this test. a) O negative result b) O positive result c) O indeterminate result Review Later d) O variable resultBelow is a diagram of the serial dilution of a culture with 1 x 10º cells. Fill out the missing information. 250 Culture Diluent 1 x 10° cells/mL 9 mL 9 mL 9.9 mL 9.9 mL 99.9 mL Volume to add to diluent Final Dilution level Theoretical count after plating 100 uLDetermine what percentage of the culture was living (viable) and what percentage was dead (mortality). Plates Plate Dilution Volume plated No.of colonies Avg No Concentration of diluted sample Cd(cells/mL) Concentration of original sample Cu(cells/mL) 1 10^-3 10ul R1=130,R2= 110,R3=210 150 150mL 1.50*10^6 Volume of cells(mL) Volume of diluent(mL) Total dilution(D) Hemocytometer count Avg cells in 1 mm^2 area Concentration of diluted sample Cd(cells/mL) Concentration of original sample Cu(cells/mL) 4.3 0.5 0.1 grid 1= 171 , grid2 = 185 178 1.78*10^5 1.78*10^6
- Create a graph of this spectrophotometric data (using Excel or the graph paper below), labeling the four stages of the growth curve- lag, log (exponential), stationary, death. Be sure to label your axes properly and give your graph a title Time Culture Sampled % Absorbance 0 min 5% 30min 7% 60min 9% 90min 15% 120min 25% 150min 37% 180min 49% 210min 50% 240min 49% 270min…Write the process/steps in Preparation of Bacillus atrophaeus culture using Blood agar plates and MacConkey agar plates. Note: This will perform the students so please write it in the easiest manner. 5-10 sentences only ThanksProvided with the following data, compute the corresponding CFU/ml of the original culture. ssume that spread plate technique was done. Show your solutions. 10-1 10-2 10-3 10-4 10-5 Plate 1 TNTC 340 132 45 19 Plate 2 TNTC TNTC 242 80 25 Plate 3 TNTC 280 90 22
- With a plate count average of 297 and a final dilution on the plate of 1:1,000 ; what is the viable number of cells in original culture (CFU/ml)?The following is a picture of Eosin Methylene Blue Agar. What does the growth on this plate indicate? ASM MicrobeLibrary.org © Cheeptham CWith liquid media such as broth, is it possible to determine if the culture is pure using the naked eye only? no yes sometimesUnanswered If you have a stock culture of Tetrahymena that has 100 cells/ml, how much stock culture would you use to make a 10 ml culture with a concentration of a 10 cells/ml? Type your numeric answer and submit Unanswered A Sul Fullscreen 14 JAN 24Provided with the following data, compute the corresponding CFU/ml of the original culture. Assume that spread plate technique was done. Show your solutions. 10-1 10-2 10-3 10-4 10-5 Plate 1 TNTC 340 132 45 19 Plate 2 TNTC TNTC 242 80 25 Plate 3 TNTC 280 90 22 2Which of the following is the a-anomer? H но H Но H2C H ОН H2C- нн ОН со 4 1 2 1 Submit 3 -OH Н ОН -OH ОН Н there is no a-anomer ОН ОН Н Request Answer H Но НО Н H2C нн ОН 2 H2C ОН -OH 4 Н ОН н н H OH -О. ОН ОН Н ОН НSEE MORE QUESTIONS