What is the CFU per mL when a 0.7mL stock suspension containing 2.1 x 10^9 CFU per ml was diluted to 4.3mL 0.1M K2HPO4-KH2PO4 buffer
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1. What is the CFU per mL when a 0.7mL stock suspension containing 2.1 x 10^9 CFU per ml was diluted to 4.3mL 0.1M K2HPO4-KH2PO4 buffer?
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- L of Microscopes: 1. If you have a standard ocular lens of 10x what is the true magnification that you are looking at if you are using the 100x objective lens. What is the rule for focusing if you switch to the 40x lens? 2. Calculate the sizes of the following cells. Not all cells are viewed at 40x so use the following table to calculate the size of your cells. 4x 1 reticular unit = .025 mm 10x 1 reticular unit = .01 mm 40x 1 reticular unit-.0025 mm 4 points A) Cell 1 on 10x=8 reticular units B) Cell 2 on 40 x = 28 reticular units mm mm Which one is actually larger? Which one is probably the plant cell? Why?Given the following summary of the two microscopes, identify the microscope to be used in these scenario: 1. An image with 18x actual magnification and an apparent size of 1.04mm 2. Unknown specimen that is 0.435 angstrom 3. A microorganism with 2.1 nm size *show solution and brief explanation2. Compute for the calibration factor. Show your solution. CF = stage micrometer divisions subtended by om x value of one stage µm division ocular micrometer divisions subtended by stage micrometer (1 su x 10μm) 10 ou CF = CF = 1μm Ocular scale Stage micrometer scale or CF = Computation: (stage units x 10μm) ocular units 60 70 80 90 100 Name of Organism: Organism A Objective Used: HPO Total Magnification: 450x Ocular Micrometer units (violet rods): 10 Length of Organism:
- FOV measured at 100x total magnification is 400 micrometers. What is the FOV at 40X total magnification? 2. If FOV is 5 mm and the number of cells in FOV is estimated at 35, what is the cell size in mm? In micrometers?You have a microscope equipped with 10 x oculars and 4 objectives, which exhibit the following characteristics (M, NA): A=(4 x/0.13); B=(20 x/0.4); C=(20 x/0.7); D=(40 x/0.65) Tick the right answers: o Objectives B and C are equivalent. o Objective D will allow more detailed observation than objective C. o To see small structures, you should choose objective D rather than C. o Objective D has a lower resolution than objective C. o To observe large objects, it is better to use objective B rather than objective CThe significance of using immersion oil when using 1OOX objective lens is The significance of using immersion oll when uskng 100X objective lens is: It will increase the contrast by having same refractive index as the lens, loss of light by refraction, reflection and diffraction is minimized. the specimens need not be killed, fixed and stained more light is captured for better illumination, allowing the specimen to fluoresce i. il. iii. iv. A light microscope should have the following features contrast Resolution magnification fluorescence ii. iv.
- Given: The used ocular objective while taking this image, has magnifying power of 6x The used objective while taking this image, has a magnifying power of 100x The used stage micrometer has spaces graduation of 01 mm each Ten (10) graduations pn the ocular micrometer conincided with two (2) graduations on the stage micrometer Questions: What is the total magnification of the image under microscope? Show your solution. What is the distance of one ocular division? Show your solution. What is approximate cell length of Cell A, Cell B, and Cell C? What is approximate combine cell length of Cell A and Cell B? Show your solution. Remember: Always measure the cell length on the longest middle section of the cell!Two microscopes are available for students to use in your laboratory, with the following specifications: Table 1. Specifications of available microscopes in the laboratory. Microscope 1 Microscope 2 Eyepiece 10x 15x Objectives 4х, 10х, 40х, 100х 10х, 15х, 40х, 100х Numerical Aperture size 0.15, 0.25, 0.65, 1.4 0.25, 0.45, 0.65, 1.25 (per objective) A. Compute for the total magnification of an image under the LPO for both microscopes. B. Calculate the A and r for both microscopes given in Table 1. Find the actual size of the specimen or provide the appropriate conversions of the given sizes as needed to determine which of the two microscopes can you use for the following: 1. an image with 18x actual magnification and an apparent size of 1.04mm 2. unknown specimen that is 0.435Å 3. microorganism that is 2.1nm in size Table 2. Summary of computed values for two microscopes available in the laboratory. Microscope 1 Microscope 2 Total Magnification (LPO only) Wavelength of light (A) Limit…Given: The used ocular objective while taking this image, has magnifying power of 6x The used objective while taking this image, has a magnifying power of 100x The used stage micrometer has spaces graduation of 01 mm each Ten (10) graduations pn the ocular micrometer conincided with two (2) graduations on the stage micrometer Question: What is the total magnification of the image under microscope? Show your solution.
- In confocal microscopy, what is the theoretical resolving power of the objective used (63X, NA 1.3, oil immersion noil=1.52)? Clearly show your calculations.2. For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much diluent must be added to the 5.3 mg/mL red cell to prepare the stock solution? Show pertinent solution/s. How much of the diluent must be added to tube 2 if 200uL Red cell suspension is added? Show pertinent solutions. What are the initial concentrations used for tubes 3, 5, and 6? Show pertinent solutions.4. If the diameter of the field of view (FOV) for your microscope is 5.5 mm at 40x total magnification, what is the diameter of the FOV at 1000X? Show work and don't forget units! 5. Use the counting method to estimate the length of an elodea cell. You will need to use your calculation for the FOV at 400x total magnification. Show work and don't forget units! Elodea Leaf at 400x total mag 6. Use the percent method to estimate the size of a human cheek epithelial cell. You will need to use your calculation for the FOV at 1000x total magnification. Show work and don't forget units! Cell Membrane Nucle us
