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- Mangaba, the laboratory technician, is interested in determining narcotics in saliva at this concentrations > 10 mg/L (μg/mL). One approach being considered is to use an immunochemical assay. Which immunoassay would be most suitable for the desired assay? Explain. Describe the immunoassays that are sensitive enough to measure as little as 10 mg/L of antigen? Which of these assays can quantitate urine albumin? Which of these assays are technically complex or require dedicated, expensive instrumentation?An injection contains 5 million international units (MIU) of interferon alpha-n3 (ALFERON N) proteins per milliliter. The recommended dose is 0.05 mL. The literature states that the activity of interferon alpha-n3 is approximately equal to 2.6 × 108international units/mg of protein. Calculate (a) the number of international units and (b) the micrograms of interferon alfa-n3 proteins administered per dose.Which of the following media is capable of detecting all clinically significant antibodies while avoiding all clinically insignificant antibodies? Question 1 options: Gel technology Tube technique with PEG No single media is capable of doing this Solid phase
- Identify three other methods commonly used to visualize lipids on a TLC plate. Specify the type or class of lipids that are detected using these methods.How would you prepare a 1:350 dilution of secondary antibody in a total volume of 3mL?In (b), why would it be more efficient to use labeled anti-human IgG rather than label the patient’s antibodies?
- Serial dilutions are made in order to determine the level of the antibody in the sample. True or False ?2. https://doi.org/10.1186/s12868-022-00692-1 (link to research) a) In the immunohistochemistry section of the materials and methods section the authors wrote “The number of positive cells in hotspot areas in ten high power fields (HPFs) in areas of demyelination and plaques in the brain stem were counted using the image analysis software (Lecia Application Suite Version 4.12.0, Welzlar, Germany).” Why were they looking at demyelination areas for this study? b) In the effect of mitoxantrone on histopathological changes in the brain section of the results section the authors wrote “Active plaques revealed inflammatory cellular infiltrates with abundant macrophages stuffed with myelin debris, an evidence of ongoing myelin breakdown.” What does macrophages stuffed with myelin debris have to do with the study?INTERPRETATION OF RESULIS NEGATIVE: Two lines appear. One colored line should be in the control region (C), and another apparent colored or faded color line adjacent should be in the test region (T). This negative result indicates that the drug concentration is below the detectable level. POSITIVE: One colored line appears in the control region (C). No line appears in the test region (T). This positive result indicates that the drug concentration is above the detectable level. INVALID: Control line fails to appear. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the procedure and repeat the test using a new test panel. If the problem persists, discontinue using the lot immediately and contact your local distrībutor. READING OF RESULTS INTERPRETATION SONTROL TEST SAMPLE
- A technologist failed to notice that the centrifuge had not properly centrifuged the test tubes prepared for antibody identification. The time of centrifugation was 15 seconds instead of 30 seconds. What would be the potential error in the interpretation of this test? can please any one provide me ans?You have 4 ml of an antigen solution, how would you prepare a 3-fold dilution series such that you will have at least 2 ml of each dilution? Please draw a picture and explain, I don't understand what it means by 3-fold. Please explain, no copy paste asapWhat is the result of the Antibody Screening Test? Based on the result of the autocontrol, it is safe to say that the antibodies which reacted with the screening cells and panel cells are?