Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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What is a recombinant DNA?
Select one:
a. During meiosis, sister chromatids cross over to produce daughter cells with recombinant DNA.
b. Any genome that is non-parental is called a recombinant DNA.
c. New DNA sequences are inserted into a genome to produce recombinant DNA.
d. Bacterial plasmid with sticky ends are referred to as the recombinant DNA.
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- If a researcher accidentally transformed the bacterial two hybrid plasmids ( pKT25-X and pUT18-Y) into a normal strain of E coli that produces adenylate cyclase, his/her experiment will: a. show a high lacZ activity in all tubes (control and experiment) because Proteins X and Y do not need to be interacting in order to activate lacZ gene prescription. b. not work because cells lack cAMP. c. need replicates in order to show true interaction between X and Y.arrow_forwardThe following features is(are) the requirement(s) for homologous recombination tooccur:A) Very similar (homologous) DNA sequences are required.B) The site of exchange can be anywhere in the homologous sequences.C) Nucleotide sequences do not alter at the site of exchange.D) Enzymes are required to cut both strands of the double helix.E) All of the above.arrow_forwardA.) Transformation is best described as: Group of answer choices The integration of foreign DNA into a genome The uptake of naked DNA from the environment The transfer of DNA via a bacteriophage Transfer of a plasmid from one organism to another B.) What is the function of the araC gene in the pGLO plasmid? Group of answer choices It encodes the protein that glows under ultraviolet light It allows us to select for the cells that contain the plasmid It prevents the transcription of the green fluorescent protein unless arabinose is added It ensures that the plasmid will be copied and passed on to daughter cells C.) What is the function of the bla gene in the pGLO plasmid? Group of answer choices It encodes the protein that glows under ultraviolet light It allows us to select for the cells that contain the plasmid It prevents the transcription of the green fluorescent protein unless arabinose is added It ensures that the plasmid will be copied and passed on…arrow_forward
- Can you please answer question 33 and 36arrow_forwardWhich of the following is NOT an example of a spontaneous mutation? A) errors in replication of DNA polymerase B) ALL of these are examples of spontaneous mutations C) covalent alteration of DNA by chemical products of fatty acid metabolism D) incorporation of a nucleotide analog during DNA synthesis E) failure to correct an apurinic site F) nondisjunction during meiosisarrow_forwardTransposition is a process in which a discrete DNA entity can move between DNA sites that lack homology using a self-encoded protein called a: a. kinase b. transposase c. mobilase d. recombinasearrow_forward
- Cells that have deregulated expression of telomerase can become cancerous. This can be attributable to what? A. Telomerase can directly induce mutations in essential genes that can result in cancer. B. Telomerase allows cells to replicate indefinitely without end-shortening issues. C. Telomerase interferes with the function of repair enzyme complexes at the ends of the chromosome, causing DNA damages to persist. D. Telomerase inhibits regulators of the cell cycle leading to cancer.arrow_forwardOnce in human cells, which of the following statements might best describe how COVID19 replicates? a) Manipulates DNA Polymerase III to create a leading strand replication of the RNA b) Manipulates DNA Polymerase III to create a lagging strand replication of the RNA c) Manipulates Reverse Transcriptase to transcribe RNA sense strand into DNA antisense strand then back d) Manipulates RNA Polymerase II to transcribe RNA sense strand into RNA antisense strand then back e) Manipulates RNA Polymerase III to transcribe ribosomal RNAs for replicationarrow_forwardThe diagram illustrating the polymerase chain reaction (PCR) technique is provided below. How does the number of copies of the DNA region being amplified change at the end of each cycle of the polymerase chain reaction? Group of answer choices a. The number of copies triples (or triplicates). b. The number of copies does not change. c. The number of copies quadruples (or quadruplicates). d. The number of copies doubles (or duplicates). e. The number of copies halves.arrow_forward
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