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- What is Chemogeny?Aeroderma pigmentosum patients have a 10,000-fold increase in the risk of developing Skin cancer. These patients have to avoid exposure to UV light. Please explain why. To answer the question please: I) name the process that is affected and provide a scheme; 2) indicate template, substrates, sources of energy that are involved in the process; 3) propose the consequences of the process.What is meant by the term “chemolithotrophy”? In what wayare chemolithotrophs like plants?
- What are the advantages and disadvantages of differential centrifugation?Explain about different types of bioreactors and how they are used in cell suspension culture. What are the advantage and limitations of each bioreactors?What are the equations for chemoautotrophic process and carbon sequestering? What is a CHP sytem?
- What is methanobactin? How bacteria acquire it? Briefly write down the working principle of methanobactin.In Methicillin resistant bacteria, what have the bacteria developed that allows them to stay alive in the presence of Methicillin?1.). a.) What particular pathways or enzymes appear distinct for sulfate reducing bacteria in order to harness energy (create ATP)? b.) What interesting structures or unique characteristics allowed sulfate reducing bacteria to solve physical and/or chemical challenges/problems in conserving energy?
- What is the basic principle of GPC (gel permeation chromatography)?Differentiate the growth curve exhibited by the same inoculum under the following conditions. Draw and label the parts of your growth curve. A). If it is exponentially growing and inoculated in the same nutritious medium under the same optimum conditions. B). If it is taken from an old culture and inoculated into the same nutritious medium and incubated under the same optimum conditions in (A). C). If it is taken from an old culture and inoculated to a different and less nutritious medium and incubated under the same optimum conditions as (A).Sydney Brenner isolated Salmonella typhimurium mutants that were implicated in the biosynthesis of tryptophan and would not grow on minimal medium. When these bacterial mutants were tested on minimal medium to which one of four compounds (indole glycerol phosphate, indole, anthranilic acid, and tryptophan) had been added, the growth responses shown in the following table were obtained. Mutant Minimal medium Anthranilic Indole glycerol acid Indole Tryptoph phosphate trp-1 trp-2 trp-3 trp-4 trp-6 trp-7 trp-8 trp-9 trp-10 trp-11 - Give the order of indole glycerol phosphate, indole, anthranilic acid, and tryptophan in a biochemical pathway leading to the synthesis of tryptophan. Indicate which step in the pathway is affected by each of the mutations. + 1 + 1 II