Given that you start with 20 copies of double-stranded DNA prior to running PCR, how many copies would you theoretically have if you did 10 rounds of PCR? 20 200 1024 20,480 163,840

How many molecules (target sequence copies) will be produced by 30 PCR cycles? Assume you start with only 1 copy of the target sequence (very unlikely)? Show your work!

Describe the method of a PCR technique in which you can amplify fragments randomly? Briefly.

fellow lab worker brings you DNA containing what might be a similar gene in Leopard Geckos (XG). She asks you to see if you can amplify it using the same primers you used in frogs and the Bearded Dragon. You run the PCR and then analyze the product by running the DNA on an agarose gel. As a control, you run out the PCR product you amplified in Bearded Dragons and a DNA “ladder” of DNA pieces of known sizes. The gel results are shown below. Marker = DNA ladder; Lane A = Bearded Dragon PCR product; Lane B = Leopard Gecko PCR results. In the box below, determine the sizes of the PCR products shown in Lanes A & B. Size of Bearded Dragon PCR product: ______________________________  Sizes of Leopard Gecko PCR products: ______________________ yes or No - based on the results shown in the gel above – would you say the frog PCR primers for gene X amplified the same gene in the Bearded Dragon?