What does next generation rna seq technology determine? difference of expression between two alleles of same gene change of stability of protein in cell post modifioon specific gene all above none above

What does next generation rna seq technology determine? difference of expression between two alleles of same gene change of stability of protein in cell post modifioon specific gene all above none above

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Description: What does next generation rna seq technology determine? difference of expression between two alleles of same gene change of stability of protein in cellpost modifioon specific gene all above none above

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter14: Biotechnology And Society

Section: Chapter Questions

Problem 7QP

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Include references! How can we analyze the interest protein expression in cultured cells after we transfect with the following vectors including gene of interest: Green fluorescent protein (GFP) including vectors His-tag including vectors
RNA sequencing can detect DNA mutations typically easier to analyze can detect transcripts with very low abundance Both Answer Bank used for gene-expression analysis Microarray requires transcript-specific probes
Which of the following biological questions could be examined by RNA-seq? The impact of temperature on gene expression enzymatic differences at different temperatures The impact of drug treatment on gene expression Gene expression differences between genotypes translation rates of different proteins The location of Pol-Il binding sites in the genome
LO65 Compare methods that Covid tests and Covid vaccines use. What should the mRNA molecule of a regular mRNA vaccine contain to be translated accurately by our cells? a-Manking UTRS b-alternative codont c-DNA template strand d-promoter e-poly A tail f- S cap asap please
For each of the features present on the plasmid (vector) indicate whether it is needed for use during clinging and replication in bacteria, GFP expression in eukaryotic cells, or not applicable: antibiotic resistance (BAC, GFP, or NA) multiple cloning sites (BAC, GFP, or NA) poly-A signal (BAC, GFP, or NA) promotor (BAC, GFP, or NA) GFP gene (BAC, GFP, or NA) origin (BAC, GFP, or NA) Pribnow box (BAC, GFP, or NA) TATA box (BAC, GFP, or NA)
Besides using vector-based methods to transform crop plants, elaborate on THREE (3) other methods to introduce gene of interest into plant cells for the production of transgenic crops.
DNA vaccines require the DNA to be transported into the nucleus where it becomes part of the genome. This is a concern because when it inserts in a chromosome it can disrupt a gene. This is not a concern for RNA vaccines. What ensures that these vaccines will not integrate into the host cell's genome? What is Ruon to the tune of Hecutacis ucad acer
What is the role of the following molecules in the CRISPR mechanism? * Sequence of 3 nucleotide Not a RNA significant sequence Endonuclease motif that is Host genetic synthesized role in information to match a a binding CRISPR target site mechanism sequence Cas9 guide RNA DNA polymerase PAM Cellular DNA TRNA O O 口ロ口ロロ

Cre-Lox system is used for site-specific modification of DNA for genetic engineering applications. The reporter gene construct shown below is used to test the Cre-Lox system. Cells are transfected with the construct shown below and the activity of the constructs is determined by visualizing the cells with a fluorescence microscope. Match the following conditions with the expected cell observations. Hint: Make sure you note the position of the Start and Stop. The GFP and RFP genes shown do not have start codons. Cre/Lox Reporter Gene ATG LoxP CMV-Pro A. No Expression GFP Stop B. GFP Only LoxP Absence of Cre Cells treated with drug that induces expression of Cre RFP C. RFP Only A. Image A B. Image B C. Image C D Image D express ? D. RFP and GFP
What kind of DNA is actually used to make the spots for analysis for the Vicrochip DNA microassay? From the following which is the best choice To make the spots conserved regions are used from known virus genomes To make the spots a mix of conserved and variable regions are used from knwon virus genomes To make the spots variable regions are used from virus genomes To make the spots conserved regions are used from known virus genomes None of the above
Please place the stages of a CRISPR-cas9 gene editing workflow in the correct order below. Note, there is one incorrect and state why? Sequence Select for correctly gene edited cells (using antibiotic resistance and/or colour production for example) Clonal isolation Synthesise DNA insert oligonucleotides Clonal characterisation (analysis of phenotype) Add green fluorescent protein gene sequence into plasmid to aid selection of correctly transfected cells. Transfect cells Clone into CRISPR-cas9 expression vector Purify plasmids Design a set of targeting sequences using algorithms.
CA Live Remote Consider the following chart: What type of mutation is this? * DNA: TAC GCA TGG AAT MRNA: AUG CGU ACC UUA Amino acids: Met-Arg-Thr-Leu DNA: TAC GTA TGG AAT MRNA: AUG CAU ACC UUA Amino acids: Met - His - Thr- Leu substitution deletion insertion frameshift mutation