How do you clone a 4 kb promoter from a genomic cell preparation?

How can transposons be used in bacterial genetics?

How can deletion experiments within gene sequences help identify important DNA sequences for the expression of a gene?  Discuss how this was done to initially identify UP elements.  What is the result of deleting an UP element?

Primer 1 primes synthesis of the 5' to 3' strand (i.e. sense strand) of an oat cDNA/gene and encodes for first few amino acids appearing at the N-terminal end of the corresponding polypeptide. Primer 156 primes synthesis of the 3' to 5' strand (i.e. antisense strand) of an oat cDNA/gene and encodes for the last few amino acids appearing at the C-terminal end of the polypeptide. The polypeptide encoded by this gene is 156 amino acids in length.   QUESTION:-Assume that a PCR using the combination of Primer 1 and Primer 156 plus oat genomic DNA resulted in the amplification of a DNA fragment of the 160bp larger than PCRs in (1) above. With respect to the structure to this relevant gene, what does this result indicate? Explain how you reached this conclusion

how to design an experiment to test weather the mutation is on enhancer, promoter, or protein-coding region?

Why is the detection of post-translational modifications important in proteomics?

What are genetic codes with suitable examples?

How do you design a reporter assay for Serpina1?

You have generated 5 different cancer cell lines in your laboratory. In cancer cell line A there is a deletion of gene A  In cancer cell line B, the promoter for gene B is over-methylated In cancer cell line C, there is a nonsense mutation near the start codon of gene C In cancer cell line D, there is a duplication of gene D  In cancer cell line E, a translocation puts gene E close to an active promoter Each of the genes above is implicated in cancer.  What is most likely, given the information?  Gene A is likely to be a:       Proto-oncogene/ Tumor Suppressor/ Neither Gene B is likely to be a:       Proto-oncogene/ Tumor Suppressor/ Neither Gene C is likely to be a:       Proto-oncogene/ Tumor Suppressor/ Neither Gene D is likely to be a:        Proto-oncogene/ Tumor Suppressor/ Neither Gene E is likely to be a:        Proto-oncogene/ Tumor Suppressor/ Neither

Design a six-residue nucleic acid probe that would hybridize with the greatest number of E. coli gene promoters.