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- What does it mean to say thhat extension by DNA polymerase III proceed 5'---3'? A. The 5' end of a DNA polymerase molecule attaches to the 3' end of primase. B. DNA polymerase adds nucleotides to a growing strand, moving in the 5' to 3' direction. C. DNA polymerase seals nicks as it moves along a DNA strand toward the 3' end. D. DNA polymerase can only synthesize DNA at the 5' end of an existing strand of DNA.a. Pfu Polymerase b.dNTPs c.Buffer Match each component above to the correct function(s) listed below. Write your selection(s) for each component. You may have more than one answer for each. 1. unwinds DNA 2. synthesizes new DNA strands 3. enzymatically catalyzes Quikchange 4. nucleotide source for new DNA strands 5. Energy source for reaction(s) 6. Repairs errors in base pair matching 7. Maintains pH and salt levels 8. Creates polymer chains5.A. Draw a wild-type replication fork showing leading and lagging strands and their 5 to 3' direction of synthesis (arrow). B. Draw a replication fork in a RNA primase mutant (no RNA primase function). C. Draw a replication fork in a DNA helicase mutant. D. Draw a replication fork in a DNA polymerase mutant that lacks the proofreading function (but possesses all the other DNA polymerase functions).
- 1. Photoreactivation destroys the covalent bond by using the light energy from the UV light source that does not occur in humans. a. True b. False Answer: 2. Single-stranded annealing can cause loss of genetic information. a. True b. False Answer: 3.) This family of enzymes removes the damaged base before a nucleotide gap can be filled by DNA polymerase. a. Ligase b. Polymerase c. Tropo-isomerases d. Glycosylases Answer:DNA polymerase with 5'-3' as well as 3'-5' exonuclease and proofreading activity is Select one: a. Telomerase b. DNA Pol-I c. DNA pol-III d. Primase2. Dr. Kim at Ewha Research Center performed shotgun Sanger sequencing on an unknown DNA sample, and obtained the following reads (12 reads). Since the length of each read is quite short, Dr. Kim ran the original sample in a gel electrophoresis, and realized that the original DNA is just 50 base pairs long. (Please note that the resolution of gel electrophoresis is not so good. Thus, we cannot figure out the exact length of DNA using gel electrophoresis in the real world.) 1) САСТС ССAGT GTACC T 3) GGAGT CAАТC GC 5) GGCTG TGCTT GG 7) GATGG CTGTG 9) CAGTG TACCT GCA 11) TGCAA GCGA G 2) AGCCG AGATG GCTG 4) CTGCA AGCCG A 6) CTTGG AGTCA ATCGC 8) САСТС ССAG 10) GCTGT GCTTG G 12) TGCTT GGAGT (a} Find the sequence of the original DNA (reconstruction), and align each read with the reconstructed DNA sequence. (hint: put all reads on a ppt slide, and move them around to find overlaps.) (b) Calculate coverage at each nucleotide position of the reconstructed DNA, i.e., how many reads cover that…
- What is the role of alcohol in extracting DNA? 1.DNA is a polar molecule with an overall negative charge, and as such is not soluble in alcohol, and therefore precipitates. 2.DNA is a polar molecule with an overall positive charge, and as such is not soluble in alcohol, and therefore precipitates. 3.DNA is a non polar molecule with an overall negative change, and as such is soluble in alcohol, and therefore precipitates. 4.DNA is a polar molecule with an overall positive change, and as such is soluble in alcohol, and therefore precipitates.34. DNA polymerase is responsible for the process of a. Meiosis b. c. Replication d. Protein synthesis Both b and c e. Proofreading 35. Helicases are a. Enzymes involved in base pairing b. Enzymes involved in protein synthesis C. Enzymes involved in all aspects of nucleic acid metabolism 36. Polymerase I catalyzes a. Fidelity and processivity of DNA replication b. DNA damage repair c. The 5' to 3' DNA polymerization 37. Polymerase II catalyzes a. Fidelity and processivity of DNA replication b. DNA damage repair c. The 5' to 3' DNA polymerization 38. Polymerase III a. Fidelity and processivity of DNA replication b. DNA damage repair The 5' to 3' DNA polymerization C. 39. DNA polymerase I processes RNA primers during lagging- strand synthesis and fills small gaps during DNA repair reactions a. True b. False 40. DNA Polymerase II is coded by polB gene. It is made up of 7 subunits. Its main role is to repair and serve as a backup of DNA polymerase III. True a. b. False 41. The replication…1. How do we describe the two strands of DNA, once they have separated during denaturation? 2. Polymerase Chain Reaction requires both forward and reverse primers. Where do each of these primers bind? 3. What is the purpose of the primers in a PCR reaction? 4. PCR separates molecules using what property of DNA? Based on this, which direction do DNA molecules travel through a gel? (to the positive end? Or the negative end?) 5. What does ‘qPCR’ stand for and how is it different from regular PCR?
- 2. Dr. Kim at Research Center performed shotgun Sanger sequencing on an unknown DNA sample, and obtained the following reads (12 reads). Since the length of each read is quite short, Dr. Kim ran the original sample in a gel electrophoresis, and realized that the original DNA is just 50 base pairs long. (Please note that the resolution of gel electrophoresis is not so good. Thus, we cannot figure out the exact length of DNA using gel electrophoresis in the real world.) 2) AGCCG AGATG GCTG 4) CTGCA AGCCG A 1) САСТС ССAGT GTACC T 3) GGAGT CAATC GC 5) GGCTG TGCTT GG 7) GATGG CTGTG 9) CAGTG TACCT GCA 11) TGCAA GCCGA G 6) CTTGG AGTCA ATCGC 8) САСТС ССAG 10) GCTGT GСТTG G 12) TGCTT GGAGT (a) Find the sequence of the original DNA (reconstruction), and align each read with the reconstructed DNA sequence. (hint: put all reads on a ppt slide, and move them around to find overlaps.) (b) Calculate coverage at each nucleotide position of the reconstructed DNA, i.e., how many reads cover that…The proofreading function of DNA polymerase involves the recognitionof a ________ and the removal of a short segment of DNA in the __________ direction.a. missing base, 5′ to 3′b. base pair mismatch, 5′ to 3′c. missing base, 3′ to 5′d. base pair mismatch, 3′ to 5′40.Would it be possible to start synthesizing the daughter DNA strand without assembling the RNA primer first? Why? Why not? A.Yes, because the 5' PO4 is already present in the DNA strand which will be used as a template. B.No, because the RNA primer which contains the free 3' OH in its ribose has to be synthesized by primase first. C.No, because the RNA primer which contains the free 5' PO4 in its ribose will not be synthesized by primase. D.Yes, because the 3' OH is already present in the DNA strand which will be used as a template.