Vernon wanted to determine the colony forming units of bacteria per gram of soil in their soil garden. He obtained 10 grams of soil from the garden and serially diluted it. He spread plated the samples of 10, 104, and 105. After incubation, the colony counts are as follows: 1:100 000 19, 13 1:1000 1:10 000 TNTC, TNTC 222, 217

A farmer working on a piece of machinery gets his shirtsleeve caught in a moving piece of the equipment. His shirt is sliced, and a sharp blade covered in mud slices through his upper arm. He attempts to control the bleeding and immediately seeks medical attention. After 3 days, he develops a fever and his arm becomes extremely swollen and painful. Pulling back the bandages, he finds that the wound has become blackened and is leaking bloody fluid. Microscopic analysis of the fluid reveals the presence of gram-positive bacilli. a. Discuss what condition the patient is suffering from and the likely causative agent of this infection. b. Explain how the patient contracted this pathogenic microbe and what virulence factors contributed to the pathogenesis seen at the wound site. c. In addition to antibiotics, the physician prescribes hyperbaric therapy. Describe what this treatment involves and how it could be therapeutic to this patient.

What is the correct genus and species of your unknown Gram (+) bacterium?

After the original specimen has been plated and a reported gram-negative rod was seen on the initial gram stain, what is your initial assumption of the organism causing this disease?