You perform several tests on an unidentified bacterial species and get the results below. Based on the test results and the bacterial table, you narrow down the identity to two possible bacteria. Which test should you now do with these bacteria to narrow down the possibilities to just one bacterial species from the bacterial table? (In other words: knowing the results from which test would let you identify these bacteria?) Gram reaction result: Citrate Test (Tube B): O Nitrate reduction test O Motility test MSA plate O Glucose fermentation test

You have used morphological observations and biochemical tests to identify two unknown bacterial isolates in your practical sessions, briefly describe three other techniques that can be used to identify unknown bacteria (you can use any reliable online resources). Note: These must not be based on biochemical tests (enzyme use, acid production etc) or morphological characteristics (size, shape, structures).

You and a partner conduct the acid-fast staining using the same two bacteria. You know that there will be an acid-fast bacteria present. Your slide shows acid-fast and non-acid fast bacteria. Your partners show both bacteria are non-acid fast. Your partner doesn’t understand why. What errors do you think occurred? Please state 2 and explain why these errors would cause this result.

Column 1 will list ALL characteristics you have identified or tested (include a Gram reaction, shape (morphology), and size). Column 2 will include the results for your unknown (the heading should read unknown #....). Column 3 will contain the results for your identified organism (taken from Bergey’s or other sources). Please provide a footnote/citation to indicate the sources of your different information. For example, How do you know that organism “Genus species” is acid from glucose +? Citations tell me where you looked that up.