Using the TABLE shown, identify which proteins would elute together in a gel filtration experiment. 1 & 2 and 4 & 5 1, 2 & 4 2 & 3 1 & 2 4 & 5

You perform a Bradford assay. You obtain the absorbance values listed below from the BSA samples; your protein sample yields an absorbance of 1.3; what is the protein concentration of your sample? How did you determine that? BSA (ug/ml) Absorbance @ 595 nm. 25 0.15 50 0.30 75 0.45 100 0.60 150 0.90 200 1.25

In this problem, you will design a filter system for a flow cytometer. The four fluorescent dyes are FITC,PE, PE-TR,and PE-Cy5. Their emisison spectra are provided below. Detectors are arranged as shown in thefigure below. The FITC signal is detected from Filter 1, the PE signal from Filter 2, the PE-TR signal fromFilter 3 and the PE-Cy 5 signal from Filter 4.   Part 4.1Provide a rationale for the order in which the filters are arranged with respect to the unfiltered signal.   Part 4.2You are given 9 candidates from which you have to choose 4 for the filters in the diagram above. Choose4 and justify your selection.# Filter type WavelengthA Long pass 650 nmB Short pass 530 nmC Long pass 490 nmD Band pass 550-590 nmE Long pass 560 nmF Short pass 640 nmG Long pass 620 nmH Band pass 620-640 nmI Short pass 590 nm

Which fractions measured from your gel filtration experiment had the most and least amount of protein detected? Which fraction likely contained the most myoglobin based off molecular weight and protein presence? Which fraction likely contained the most egg albumin based off molecular weight and protein presence? Which samples measured from your ion exchange experiment had the most protein detected? Calculate the approximate pI of your proteins using the ion exchange Table 2. Ion Exchange Samples Tested by Bradford Assay   Concentration BSA (μg/mL) Concentration BSA (mg/mL) Volume of Sample added to Bradford Reagent (μL) Absorbance at 595 nm Standard 1 250 0.25 50 0.32 Standard 2 125 0.125 50 0.16 Standard 3 62.5 0.0625 50 0.08 Standard 4 31.25 0.03125 50 0.04 Standard 5 15.625 0.015625 50 0.02 Blank 0.00 0.00   0.00   Table 3. Gel Filtration Fractions Tested by Bradford Assay Fraction #:…

You are determining the concentration of your culture, and have counted the following on your hemocytometer as shown in Figure below: (note, blue cells have taken up Trypan blue & thus are dead. Red x's are cells that should not be counted because touching one of 2 sides of square). If your cells were diluted 1:2 before counting, what is the concentration of cells in your original culture?

What is meant by the statement “orthogonal analysis methods should be used for characterization of impurities”? Why do bacterial and plant cells require partial destruction prior to osmotic lysis? How can the partial destruction be achieved?

Gradient PAGE uses differing gel concentrations along the length of the gel to achieve optimalseparation of proteins of a wide range of molecular weight proteins. How does the staining pattern of these types of gels differ from nongradient PAGE?

In the extraction of DNA from a Banana, alcohol is added to the filtrate after filtration. What will happen if you do not filter the solution inside the zip lock prior to addition of cold alcohol?

Which of the following correctly matches the chromatography technique with the molecular property being exploited for separation? ion-exchange: charge,affinity: polarity, gel-filtration: molecular mass ion-exchange: charge, affinity:molecular binding preference,gel-filtration: molecular size ion-exchange: charge,affinity: molecular binding preference,gel-filtration: isoilectric point ion-exchange: ion binding preference,affinity: molecular size,gel-filtration:polarity

The diagrams below show the results of three FRAP experiments. Match the proteins to the statements. Protein B 1.0 0.8- 0.6- EVL 0.4- 0.2- 50 100 150 time (seconds) 0.2- 50 Protein A 100 150 time (seconds) 200 250 Protein that is completely tethered/anchored and does not move in the memebrane Protein that has some of it moving slowly while others are tethered/anchored in the membrane Protein that is fully mobile in the membrane Represent proteins associated with the cell membrane Represent cytoplasmic proteins that are moving around [Choose ] [Choose ] A, B and C None of these A Both A and B B с [Choose ] [Choose ] [Choose ] 200 250 50 Protein C 100 150 time (seconds) T 200 250