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You have determined that a cell suspension contains 1 x 103 cells/mL. That cell suspension was prepared by adding 1 ml of a more concentrated cell suspension to 999 mL of phosphate buffered saline. What is the concentration of cells in the original cell suspension?
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- After blood collection, the red cells are separated from the serum to be used for the preparation of the stock solution. How is it done? For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much diluent must be added to the 5.3 mg/mL red cell to prepare the stock solution? Show pertinent solution/s. What is the appropriate diluent used for the preparation of the red cell suspension?If a red blood cell was placed in a 50mOSM solution, the cell would:Your supervisor gave you a 5% saline (NaCl) solution. You need to make 100ml of 1.0% saline solution. How much 5% saline would you take to complete the task?
- If you made a hematocrit in two tubes. Tube A is made of 0.60M NaCl, and Tube B is made of 1.00M of NaCl, describe how the cell would respond in these solutions? Which tube would have a lower hematocrit?Isotonic, hypotonic, and hypertonic IV (intravenous) fluid solutions are widely used in the healthcare setting. As a possible health care professional, you must know how each of the solutions work on the body and why they are given. a) When does each type of solution must be applied to a patient? b) What does each type of solution do the cells? Isotonic IV fluid solution Hypotonic IV fluid solution Hypertonic IV fluid solution|I am trying to figure out how much albumin and globulin are in our egg white sample. The egg white is 38.51g. It was diluted with 200 mL of water, and I was told to find the dilution factor. We were told that 38.5g is equal to 1mL of water. The dilution factor I calculated was 38.51mL (egg white)/238.51mL (total volume)= 1:6 dilution factor. We then took 40mL of the diluted sample and centrifuged it to separate the albumin and globulin. Using a given standard curve, we calculated the concentrations of albumin and globulin for each tube with different amounts of each protein. There were other solutions added to each tube for the spectrophotometer, but each one had a total volume of 5mL including the albumin and globulin. To find mg protein in the tube, do I multiply the concentration by the total volume in the tube or by the mL of protein added to each tube? To calculate the amount of each protein in the egg white, I multiplied each concentration in the table by the dilution factor,…
- Mannitol, a carbohydrate, is supplied as a 25% (w/v) solution. This hypertonic solution is given to patients who have sustained a head injury with associated brain swelling. (a) What volume should be given to provide a dose of 70. g? (b) How does the hypertonic mannitol benefi t brain swelling?In making red blood cell suspension, why do we need to wash red blood cells in saline?You make 100 ml of a stock solution for mounting sections, that contains 0.5g gelatin. You take 10 ml of this stock solution and add it to 990 ml 0.1 X phosphate buffered saline. The concentration of gelatin in this diluted solution is: 0.5% 0.05% 0.005% 0.0005% 0.00005%
- 4 mL of 10% TCA solution was added to 1 mL of serum and after mixing, it was waited for two minutes and filtered through non-phosphorus filter paper. 1 mL of the filtrate was taken and 13 mL of distilled water, 4 mL of sulfomolybdic acid and 2 mL of dilute SnCl2 solution were added and mixed, and after waiting for 15 minutes, the absorbances of the obtained solutions against pure water at 520 nm were read. If the function of the calibration graph obtained with 0.5-2.5 mg/mL standard phosphorus solutions is y= 0.245x + 0.107 and the absorbance value of the serum sample is 0.109, what is the amount of phosphorus in the sample?We’re back in the lab having fun! Our current experiment calls for us to treat our cells with THC (yeet!, delta 8 from hemp of course lol) and the final concentration of 15 µM once it has been added to the cells. We need to treat 10 mL of cells, and we don't want our treatment volume to be more than 10 µL per 1 mL of cells. What concentration should we make our stock solution? (I submitted this question before and got an answer of 150 µM and that was incorrect)Given this, if you used 6g of vitamin Z powder to make 20 ml of solution, what is the % concentration of this solution? (I gave the image since I don't know if that info is needed to solve this question.)It also gives a follow-up, if you can help here too: You work in a lab as a summer student. One of your tasks is to make sure that there is enough cell culture medium containing antibiotics to grow bacteria. One day you realize that there is only 5 ml of 10% Antibiotic stock solution in the freezer. You decide to use it all to prepare the working culture medium with 0.01% antibiotic. In the lab there is plenty of growth medium without antibiotics. (Note: dilution in medium is like dilution in water). You remember the equation to make dilutions of stock solutions. You usually use this formula to calculate the required volume of a stock solution, but you realize it can apply here as well, even though the unknown is the final volume. So, you make that dilution. Given that each bacterial…