Use the data below to calculate the colony forming units per mL (CFU/mL) from a sample of bacteria after growing the sample in an incubator for 2 hours. Culture density (CFU/mL) = Time 1 hour Dilution (before plating) 1x 10¹¹ 1x 10-² 1x 10-³ 1x 104 2 hours 1x 10-¹ 1x 10² number of colonies (dilution) x (volume plated, in ml) 1x 10³ 1 x 104 Number of colonies observed Volume spread on plate (replicate plates) 0.1 mL 0.1 mL 0.1 mL 0.1 mL 0.1 mL 0.1 mL 0.1 mL 0.1 mL TNTC 660 101 8 TNTC 220 15 3 TNTC 783 115 7 TNTC 180 26 9 7

Human Anatomy & Physiology (11th Edition)
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Use the data below to calculate the colony forming units per mL (CFU/mL) from a
sample of bacteria after growing the sample in an incubator for 2 hours.
Culture density (CFU/mL) =
Time
1 hour
2 hours
1x 10-¹
1 x 10-²
1x 10-³
1x 104
number of colonies
(dilution) x (volume plated, in mL)
Dilution (before plating) Volume spread on plate (replicate plates)
1x 10-1
1x 10-²
1x 10-³
1 x 104
0.1 mL
0.1 mL
0.1 mL
0.1 mL
Number of colonies observed
0.1 mL
0.1 mL
0.1 mL
0.1 mL
TNTC
660
101
8
TNTC
220
15
3
TNTC
783
115
7
TNTC
180
9
7
Transcribed Image Text:Use the data below to calculate the colony forming units per mL (CFU/mL) from a sample of bacteria after growing the sample in an incubator for 2 hours. Culture density (CFU/mL) = Time 1 hour 2 hours 1x 10-¹ 1 x 10-² 1x 10-³ 1x 104 number of colonies (dilution) x (volume plated, in mL) Dilution (before plating) Volume spread on plate (replicate plates) 1x 10-1 1x 10-² 1x 10-³ 1 x 104 0.1 mL 0.1 mL 0.1 mL 0.1 mL Number of colonies observed 0.1 mL 0.1 mL 0.1 mL 0.1 mL TNTC 660 101 8 TNTC 220 15 3 TNTC 783 115 7 TNTC 180 9 7
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