How would you identify this unknown bacteria using a flowchart and the bacteria below as a possible unknown? Bacillus cereus, Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis and Lactococcus lactis. Create flow chart (dichotomues) using at least 3 other biochemical tests from the following list: Mannitol salt agar, Blood agar, Starch agar, Tributyrin Agar, Gelatin, Casein agar, Indole Production, MR-VP, Citrate, Hydrogen Sulfide test, Urease test, Nitrate reduction test, Catalase test, and Oxidase test.

How would you identify this unknown bacteria using a flowchart and the bacteria below as a possible unknown? Bacillus cereus, Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis and Lactococcus lactis. Create flow chart (dichotomues) using at least 3 other biochemical tests from the following list: Mannitol salt agar, Blood agar, Starch agar, Tributyrin Agar, Gelatin, Casein agar, Indole Production, MR-VP, Citrate, Hydrogen Sulfide test, Urease test, Nitrate reduction test, Catalase test, and Oxidase test.

Biomedical Instrumentation Systems

1st Edition

ISBN:9781133478294

Author:Chatterjee

Publisher:Chatterjee

Chapter19: Biomedical Laboratory Instrumentation

Section: Chapter Questions

Problem 2RT

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You must write up your OWN dichotomous key for all the possible unknown organisms listed below. The first step of the key will be the Gram Stain and the second will be Gram negative. Subsequent steps will include biochemical tests only. Solve the identity of an unknown bacterial specimen by creating a dichotomous key and using the staining, culturing and biochemical identification procedures . Possible Organisms Alcaligenes faecalis Enterobacter aerogenes Enterococcus faecalis Escherichia coli Proteus vulgaris Pseudomonas aeruginosa Salmonella arizoniae Staphylococcus aureus Staphylococcus epidermidis Staphylococcus saprophyticus Streptococcusbovis Streptococcus pyogenes. Can you please help me find the dichotomous key . Thank you !
Create a flow chart to perform the required tests to identify unknown microorganism: - Use at least 3 other biochemical tests from the following list: Mannitol salt agar, Blood agar, Starch agar, Tributyrin Agar, Gelatin, Casein agar, Indole Production, MR-VP, Citrate, Hydrogen Sulfide test, Urease test, Nitrate reduction test, Catalase test, and Oxidase test. The Gram positive organisms that could be present in your unknown mixture are: Bacillus cereus, Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis and Lactococcus lactis.
You must find up your OWN dichotomous key for all the possible unknown organisms listed below. The first step of the key will be the Gram Stain. Subsequent steps will include biochemical tests only. Solve the identity of an unknown bacterial specimen by creating a dichotomous key and using the staining, culturing and biochemical identification procedures . Possible Organisms Alcaligenes faecalis Enterobacter aerogenes Enterococcus faecalis Escherichia coli Proteus vulgaris Pseudomonas aeruginosa Salmonella arizoniae Staphylococcus aureus Staphylococcus epidermidis Staphylococcus saprophyticus Streptococcusbovis Streptococcus pyogenes Note : The test must be performed using any of this test Mannitol Salt Agar, DNAse test, Catalase test, coagulase test, Novobiocin Test, BILE ESCULIN AGAR (BEA) TEST, Oxidase test, Bacitracin Sensitivity, Blood Agar . Macconkey, Nitrate Reduction test. Thank you so much in Advance !
You must write up your OWN dichotomous key for all the possible unknown organisms listed below . The first step of the key will be the Gram Stain. Subsequent steps will include biochemical tests only. Solve the identity of an unknown bacterial specimen by creating a dichotomous key and using the staining, culturing and biochemical identification procedures . Possible Organisms Alcaligenes faecalis Enterobacter aerogenes Enterococcus faecalis Escherichia coli Proteus vulgaris Pseudomonas aeruginosa Salmonella arizoniae Staphylococcus aureus Staphylococcus epidermidis Staphylococcus saprophyticus Streptococcusbovis Streptococcus pyogenes Imp Note - the test should be performed using any of the following tests stated below. Bile Escalin test, Oxidaze test, Blood Agar , Catalese Test, Mannitol salt agar . Thank you !
Lactose fermentation Negatíve Salmonella Positive Escherichia coli Shigella Citrobacter Serratia Klebsiella Proteus Enterobacter Phenylalanine deaminase activity Indole production Negative Salmonella Positive Positive E. coli Proteus Shigella Serratia Negative Citrobacter Klebsiella H,S production Enterobacțer Negative Serratia Salmonella Shigella Positive Motility Negative Klebsiella Positive Citrobacter Citrate utilization Enterobacter H2S production Positive Negative Shigella Serratia Negative Enterobacter Positive Citrobacter
S aureus Styphimurium S aureus S typhimurium Nutrient Agar Plates MacConkey Agar Plates S epidermidis Unknown S epidermidis Unknown S aureus S typhimurium S aureus S typhimurium Mannitol Salt Agar Plates Gram Stain S epidermidis Unknown S epidermidis Unknown
Salmonella-Shigella (SS) agar plate results (kitchen sink drain and kitchen dish cloth) Using the following information about SS agar and the image below, describe and identify the growth on the SS agar plate in the table on page 6. SS agar plate - results of kitchen sink drain and kitchen dish cloth (plate below) Salmonella-Shigella (SS) agar: Salmonella-Shigella agar (SS agar) is a selective and differential medium utilized for the selection and characterization pathogenic enteric bacteria. SS agar contains lactose, bile salts, ferric citrate, and neutral red. Kitchen The bile salts select for Gram-negative bacteria, while inhibiting Gram-positive bacteria. sink drain The other components differentiate among Gram-negative bacteria. Coliform bacteria such as E. coli will ferment the lactose in the media, resulting in bacterial growth with a pink color. (They do not produce any hydrogen sulfide.) Members of the genus Salmonella will not ferment lactose, but do product hydrogen sulfide…
Discuss the correct laboratory technique in using the centrifuge for qualitative analysis. Give two tips on its proper use. Explain how calcium ions are confirmed present in a solid salt by using flame test. Discuss a method to confirm the presence of a) phenol and b) ketone in a test compound. How are proteins confirmed present in a sample? Explain the laboratory process.
Answer the following questions (not more than 5 sentences/question). Discuss the correct laboratory technique in using the centrifuge for qualitative analysis. Give two tips on its proper use. Explain how calcium ions are confirmed present in a solid salt by using flame test. Discuss a method to confirm the presence of a) phenol and b) ketone in a test compound. How are proteins confirmed present in a sample? Explain the laboratory process.
Describe characteristics of Streptococcus Agalactiae in the Agar: (How does colonies look like (color) and explain does it grow on that agar. (Don't have to write the incubation period) ~ Only describe how would it look like on the Agar: Blood Agar (Aerobic) MacConkey EMB PEA Mannitol Salt Agar Chocolate Agar Nutrient Agar
The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial dilutions were performed and a 0.1 mL aliquot from each dilution was spread onto Plate Count Agar (PCA). The number of bacterial colony forming units (CFU) after overnight incubation are shown as listed in the table below. What is the number of colony forming units per mL of the culture broth? Choose only the most appropriate plate for your calculation. Give your answer as the number only (do not add text for the units). You may use scientific notation with the format 1.12e+6 (that is, 1.12 x 106 cfu/mL). (Note: Canvas will then display your answer a whole number.) Plate 1 10 Plate 2 10 Plate 3 10 dilution dilution dilution Plate 4 10 dilution Plate 5 107 dilution Plate 6 10 dilution -6 *Too many to count Number of colony forming units (CFU) TMTC* TMTC* 840 28 19 1
The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial dilutions were performed and a 1 mL aliquot from each dilution was mixed with warm molten agar and poured into a Petri dish. The numbers of bacterial colony forming units (CFU) after overnight incubation are shown in the table below. What is the number of colony forming units per mL of the culture broth? Choose only the most appropriate plate for your calculation. Give your answer as the number only (do not add text for the units). You may use scientific notation with the format 1.12e+6 (that is, 1.12 x 106 cfu/mL). (Note: Canvas will then display your answer a whole number.) Plate Dilution Plate 1 10 dilution Plate 2 10 dilution -5 Plate 3 10 dilution Plate 4 10 dilution Plate 5 107 dilution -8 Plate 6 100 dilution *Too many to count Number of colony forming units (CFU) TMTC* TMTC* TMTC* 867 154 18