• TEST done and RESULT • MOLISCH • SELIWANOFF • BENEDICT • BARFOED • LUGOL'S - • OZAZONE TEST IODINE • Violet ring • Light yellow • Light blue • Light blue soln. • Blue • No crystallization solution soln. solution • Violet ring • Cherry red soln. • Light blue • Red soln. in 1 • Brownish • No crystallization min. solution • Cherry red soln. in 15 min. • Violet ring • Light blue • Light blue soln. • Brownish • No crystallization soln. solution • Violet ring • Light yellow • Brick red soln. • Red soln. in 1 • Brownish • Needle like solution min. solution structure • Violet ring • Light yellow • Brick red soln. • Light blue soln. • Brownish • Sunflower shape solution solution structure
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- Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE)1. What are the functions of the sample buffer and sample reducing agent? Why do the samples need to be heated before you load them on the gel?2. What determines the current in your gel? What could cause the current in your gel unit to be lower than expected? Is there anything that could cause it to be higher than expected?3. Why do you need to wash the gel before staining it? Why use warm water?SDS-PAGE reagents that play a role in formation of the gel only are(Select all that applies) Beta-Mercaptoethanol Bromophenol blue APS Heat Sodium Dodecyl Sulfate Bis-acrylamide Acrylamide TEMEDAll recipes are given per litre unless specified. Lambda Broth (LB) Plates Top Agar 10 g tryptone 10 g Tryptone 2.5 g NaCl 2.5 g NaCl 15 g Agar 7 g Agar Make 5 mL aliquots, then autoclave. Lambda Broth (LB) Media Suspension Media 10 g tryptone 5.0 g NaCl 2.5 g NaCl 2 g MgSO4· 7H2O 50 ml 1 M Tris pH 7.5 In LB medium…
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