In a 0.1000 M acetic acid solution at 25 degrees celsius , the acid ionizes to the extent of about 1.34 %. Since each molecule of acetic acid which ionizes produces 1 H+ ion and 1 C2H3O2- ion, the concentration in the solution are: HC2H3O2 < -----------> H+ + C2H3O2-
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- In a 0.1000 M acetic acid solution at 25 degrees celsius , the acid ionizes to the extent of about 1.34 %. Since each molecule of acetic acid which ionizes produces 1 H+ ion and 1 C2H3O2- ion, the concentration in the solution are:
HC2H3O2 < -----------> H+ + C2H3O2-
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- The simple form of |Hoff equation is: II = [B]RT In this equation the [B] is the molar concentration of solute. So: n m [B] = v MV = cg /MA Where c, the mass concentration of the solute is in the total volume of solution and M, is the molar mass of the solute. This equation can be replaced in the previous one to get: RT II = MA In this equation molar mass of given solute can be detemined from the slope of the II vs Cz plot. This equation applies only to solutions that are sufficiently dilute to behave as ideal-dilute solutions. In the case of non-ideal solutions, however, the extended formula is: II = [B]RT{1+ k. [B] + n. [B]² + ...} Biological macromolecules dissolve to produce solutions that are far from ideal, but we can still calculate the osmotic pressure by assuming that the van't Hoff equation is only the first term of a lengthier expression: II [B]RT(1+ b. [B]) II = RT + bRT. [B] [B] II = RT + bRT./M. */Ma п RT ÞRT Ca MA MA In this equation molar mass of given biomolecule can…A 100 ml of a 1% sol'n of boric acid (freezing point = -0.29) isotonic w/ blood by NaCl (freezing point = -0.58). what grams is needed to make it an isotonic? what weight of NaCl is needed to a 2% sol'n of atropine sulfate (E=0.13) made isotonic with blood plasma?Calculate the effective quantity (g) of sodium chloride related to tonicity in 100ml of an intravenous fluid labeled "5% dextrose in 0.45% sodium chloride," and indicate whether the solution is isotonic, hypotonic or hypertonic. The answer is Hypertonic and 1.35g NaCl but I don't understand how to get the answer.
- At what pH does a lysine solution exhibit the highest buffering capacity? (Lysine pKas: pk1 = 2.2, pK2 = 8.95, pK3 = 10.5) %3D O рH 12.1 О рH 9.72 O pH 5.67 pH 8.95When 2.43 g of a nonelectrolyte solute is dissolved in water to make 435 mL of solution at 25 °C, the solution exerts an osmotic pressure of 895 torr. What is the molar concentration of the solution? concentration: 0.045 M Incorrect How many moles of solute are in the solution? moles of solute: 0.034 mol Incorrect What is the molar mass of the solute? molar mass: 69.12 g/mol IncorrectWe have isolated a mixture of 2 protein of the same molecular weight but different isoelectric points. The isoelectric points are 7.5 for Protein A and 9.0 for Protein B. What would be the order of elution from a cation exchange column if a buffer of pH 7 is used? Group of answer choices Both proteins will remain in the column. Both proteins will elute simultaneously Protein B followed by Protein A Protein A followed by Protein B
- If several like-charged proteins are bound simultaneously to an ion exchange column, they can be separated by gradually increasing the salt concentration (applying a “gradient”). A CM-Sepharose column has three proteins bound to it: A (pI = 7.9), B (pI = 7.4), and C (pI = 8.7). At pH = 7.0, the salt concentration on the column is gradually raised from 0 to 500 mM. In what order will the proteins elute? Explain.The order of elution of amino acids X, Y, VW and Z in a cation exchanger eluted with a buffer of increasing pH is as follows: Z, W, Y, X (last). If the amino acids are glutamic acid, arginine, phenylalanine and alanine, what amino acid can Z be? O A O F O E ORPolymer beads (resin) made of DEAE (diethylaminoethyl) cellulose are packed in an ion exchange column. The total mass of beads in the column is 8.47 kg. On average, each bead weighs 0.0023 g and has an average of 18.4 * 10° positively charged amine groups that can adsorba negatively charged protein that passes through the column. A solution containing 2.07 mg/L of a protein is maintained at pH 6.3 and is passed through the ion exchange column at 0.215 L/min. The protein has a molecular weight of 154,000. The pk, of the amino groups on DEAE cellulose is 7.1, and the pl of the protein is 5.6. 2. A. How long can the column be operated before reaching 80% capacity (i.e., 80% of the amino groups on DEAE are bound to the protein through an ionic bond)? You may assume that one protein attaches to one + charge on the beads (although it's possible that proteins attach to more than one + charge). B. After reaching 80% capacity, explain what you would do to release the protein attached to the…
- Calculate the effective quantity (g) of sodium chloride related to tonicity in 100 mL of an intravenous fluid labeled "5% dextrose in 0.45% sodium chloride," and indicate whether the solution is isotonic, hypotonic, or hypertonic.A solution of Na2CO3 contains 53g of solute in 200ml of solution. What is the molarity of this solution.19. 150 mCi of D2O is injected in your patient. 10% of D2O is excreted. The concentration of D2O in a plasma sample is found to be 0.4 mCi/100 ml. What is your patient' s Total Body Water in L? 20. the diffusion coefficient states that: A. the larger the molecular radius of the solute, the slower the rate of diffusion B. the more viscous the solution, the slower the rate of diffusion C. the smaller the molecular radius of the solute, the faster the rate of diffusion D. the more viscous the solution, the faster the rate of diffusion E. the larger the molecular radius of the solute, the faster the rate of diffusion 21 You are experimenting on diffusion rates in lab. Down the center of Beaker A is a semipermeable membrane. On one side of the membrane in Beaker A is a 100mM solution of CaCl2; on the other side is pure water.Down the center of Beaker B is a semipermeable membrane. On one side of the membrane in Beaker B is a 10mM solution…