Triton X-100 is a detergent which is not commonly used to separate integral proteins from the eukaryotic cell membrane because: Triton X-100 causes denaturation of these proteins Triton X-100 is more harsh than sodium dodecyl sulfate (SDS) Triton X-100 contains polar, charged regions Triton X-100 contains polar but uncharged regions all of the above

I am not understanding why TA of alcohol precipitation is used as TA of purified protein. I provided 2 photos, 1 with the questions and the correct answer, and the other photo is a screenshot of the explanation.

Gram staining is a common technique used to differentiate two large groups of bacteria based on their different cell wall constituents. The Gram stain procedure distinguishes between Gram positive and Gram negative groups by coloring these cells red or violet. Gram positive bacteria stain violet due to the presence of a thick layer of peptidoglycan in their cell walls, which retains the crystal violet these cells are stained with. Alternatively, Gram negative bacteria stain red, which is attributed to a thinner peptidoglycan wall, which does not retain the crystal violet during the decoloring process - s. epidermidis a gram-positve coccus - E. coli a gram-negative bacillus  - C. xerosis a gram-positive bacillus  How does each organism fulfill a specific niche based on the information provided above?

What does -log(p-value) tell in immunoprecipitation mass spectrometry? What does log2 Fold change say? Generally, what does the graph of immunoprecipitation mass spectrometry tell (ie. how to read its graph)?