Under the microscope, the fluorescent signal from an isolated quantum point will form a stain: A.Approximately the same size as a fluorescent protein of the same colour B.Much smaller than a fluorescent protein, regardless of colour C.Relatively dark compared to antibody D.Which corresponds approximately to the size of the quantum point, 20 to 40nm.

In the more common protocol for immunofluorescence detection of cellular proteins, an investigator uses two antibodies. The first binds specifically to the protein of interest. The second is labeled with fluorochromes for easy visualization, and it binds to the first antibody. In principle, one could simply label the first antibody and skip one step. Why use two successive antibodies?

How would you prepare a 1:350 dilution of secondary antibody in a total volume of 3mL?

hypothetically speaking, if a rapid test was being made to detect RSV (RSV antigen), the conjugate pad would contain the anti-RSV antibody conjugated with HRP. The test line would be the anti-RSV antibody. What would be a suitable control antibody to use?