The picture shows bacteria (Escherichia coli) under a microscope Do you bacterial growth or no ( negative and positive) And explain? (Is there growth or not?)
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- In the space provided below, give me the word(s) that would correctly complete each sentence: A) The equation "d = A/ (2 NA)" describes the of a lens system B) In the flagella stain, the mordant makes bacterial flagella easier to see by making themThe image is showing Bacillus subtillis bacteria under 400x magnification, the same magnification used on the plant and animal photos. Why are the bacterial cells so much harder to see in this microscope image?(Two part question I have separated for clarity) Which stains are used to differentiate between the cell walls of bacteria? What CAN”T the Gram stain tell you about a bacteria?
- A student has a compound microscope equipped with 10X ocular lenses and 4X, 10X, 40X and 100X objective lenses. The student has a slide with cells that are -100 microns (1 micron = 10 mm) in diameter, and wants to viewa magnified image of a cell so that the cell appears to be 10 mm in diameter. Which objective lens should the student use? 10X O 40X O 100X 0 4XUsing an appropriate lens and oil immersion, what would be a microscope's maximum resolution (in micrometres) that could use a wavelength of 350 nm (assuming that such a device exists)? What would be the surface-to-volume ratio for the coccus-shaped bacterium twice the size of the maximum resolution you calculated? Why is the surface-to-volume ratio an important biological factor. Explain. Show all of your calculations.A student has a compound microscope equipped with 10X ocular lenses and 4X, 10X, 40X and 100X objective lenses. The student has a slide with cells that are -100 microns (1 micron = 103 mm) in diameter, and wants to view a magnified image of a %3D cell so that the cell appears to be 10 mm in diameter. Which objective lens should the student use? O 10X O 40X O 100X O 4X Question 7 2 pts
- a. What can you observe in viewing your stained bacterial slide under the microscope if you fixed a lot of bacterial colonies in your slide during smear preparation? b. What can you observe in viewing your stained bacterial slide under the microscope if you heat fixed your slide in a much longer exposure to heat?Describe in detail all the steps needed to determine whether an Unknown bacteria has a Gram-positive or Gram-negative cell wall using the Gram stain. (You can describe the method presented in the Exercise 3-6 Gram stain videos or in the Lab Manual.) 1. Start by describing the method of transferring the bacteria onto the microscope slide, then describe heat-fixing the emulsion of the Unknown bacteria. (Do not describe all the steps needed to perform aseptic technique. Just say “Bacteria was added aseptically.”) 2. Describe the Gram stain in detail. (This description should include all the stains, how long the stain should set on slide, and Genus and species names of bacteria you are using as CONTROLS.)Suppose you are staining three identical smears of the same bacterial culture with three different basic simple stains: carbol fuschin, crystal violet and methylene blue. You observe each stained preparation under oil immersion at 100X. which of the following bacterial characteristics would you expect to be different between each of the stains as you view them under the microscope. Bacterial arrangement Bacterial shape Size of cells The color of the bacteria
- Which of the following is never useful for observing living cells? O phase-contrast microscope scanning electron microscope O darkfield microscope O brightficld microscope confocal microscopeThis is a compound light microscope O dark-field illumination microscope O scanning electron microscope transmission electron microscope 71°F Sunny 立Assume you are observing the diatom pictured in Figure 1 using the 10X lens in a compound light microscope. You move to the 40X lens and then again to the 100X lens by only rotating the turret (remember that the lenses are parfocal), without making any other adjustments to the microscope. c) After making your adjustments, you notice that the midline of the diatom is in focus while the remainder is blurry. Explain, based on microscopy principles, why this has occurred Provide a description of the procedure to prepare an acidic stain of bacteria using Nigrosin as it would appear in the Methods section.