Two part question I have separated for clarity) Which stains are used to differentiate between the cell walls of bacteria? What CAN”T the Gram stain tell you about a bacteria?
Q: Which type of microscope(s) would be used to examine the following specimens? Explain each of your…
A: 1. Atomic force microscope : The atomic force microscope has formed into a useful asset in primary…
Q: What is the importance of Gram-staining?
A: Introduction : Gram Staining, Commonly Known As Gram's Method, Is A Staining Technique That Is Used…
Q: . How do Gram-positive and Gram-negative bacteria differ? Question 1 options: the presence or…
A: To find: How the gram negative and gram positive differ from the following options.
Q: hich Is The More Important Step In Gram Stain?
A: Hans Christian Gram invented the Gram stain in 1884. (Gram,1884).
Q: An acid-fast staining method was performed on a bacterial smear using the dyes carbolfuchsin and…
A: Introduction: Acid-fast staining is a type of differential staining technique that was first…
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Q: Are the structural differences between the cell walls of Gram-positive and Gram-negative bacteria…
A: The gram-positive and negative cells differ in their cell wall composition.
Q: Why is oil immersion necessary to see bacteria in the laboratory?
A: Oil immersion technique is used to increase the resolving power of microscope. Both the objective…
Q: Which bacterial morphology is demonstrated by this simple stain? choose as many as are correct
A: For all types of bacterial cells, a simple staining method may be used to give contrast to the…
Q: What is the importance of Gram-staining
A: Gram staining was created by Christian gram Bacteria with Peptidoglycan in their cell walls are…
Q: The picture shows bacteria (Escherichia coli) under a microscope Do you bacterial growth or no (…
A: Escherichia is a gram-negative bacterium, which under the microscope is shaped like a rod with a…
Q: Why is it important to perform a gram stain for any type of bacteria? After performing the gram…
A: The gram staining is a procedure that is useful in classifying the bacteria based on retention of…
Q: a. After conducting the Gram staining procedures and you observed that all cells are stained with…
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Q: What does the Gram stain reveal about a cell?
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A: Cell staining is a technique that is used in microscopy where certain chemicals can stick to…
Q: When doing a gram stain in microbiology, one step can be eliminated and still allow distinction…
A: Please follow step 2 for detailed explanation.
Q: You are performing a Gram stain on Gram-negative bacteria, and you stop after the addition of the…
A: Gram negative bacteria: these bacteria are enclosed in hard capsule and don't retain the crystal…
Q: what kind of microscope is used for cellular structure and why?
A: Microscopes are scientific instruments used to investigate items that are too small for the naked…
Q: plain Why do acidic dyes not stain bacterial cells?
A: Staining is the process of changing the colour of a substance to make it easier to examine tissues,…
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A: Q. How is true microbial motility distinguished from other kinds of motion like Brownian movement…
Q: What is a step-by-step process of preparing and inspecting a microorganism under a microscope?
A: Microbiology is the study of microorganisms that are invisible to the naked eye. The microorganisms…
Q: explain the differences between the Gram-negative and Gram-positive bacteria cell wall structure and…
A: Gram staining: It is a widely employed method to identify and differentiate two large groups of…
Q: If you forgot to apply the safranin counterstain while performing a Gram stain, which outcome would…
A: Gram staining is a staining technique used to differentiate two large groups of bacteria based on…
Q: If the decolorizing step was omitted in the Gram-staining procedure, what color would you expect…
A: Gram staining is performed on the basis of differential characteristics of the cell wall in…
Q: cannot be determined using the details of Gram staining process? Question 7 options: bacterial…
A: Gram staining technique is the technique which helps to differentiate between gram positive and gram…
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A: As per the honour code, we are entitled to do only three subparts of a multipart question. So, i am…
Q: a. What can you observe in viewing your stained bacterial slide under the microscope if you fixed a…
A: The staining is a very important process in microbiology that is used to identify specific…
Q: How would the slide appear if the bacteria were stained with safranin, a basic red dye?
A: The appearance of bacteria, when stained with safranin, is to be determined.
Q: nim. "Are you heading to the lab right now?" asked Kyle. "Yes." she said. “I want to look over the…
A: Gram staining procedure is used to differentiate between Gram Negative and Gram Positive Bacteria.…
Q: If the decolorizing step was omitted in the Gram-staining procedure, what color would you expect…
A: Gram staining helps in differentiation between gram positive and gram negative bacteria. Procedure…
Q: what does gram positive cells look like?
A: Introduction Gram Staining is very common and popular method for classification of Bacteria.…
Q: When performing a Gram stain on a Gram-negative bacteria, why does the bacteria stain pink? Crystal…
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Q: Which type of stain would be the best choice for negative staining of most bacterial cells: a basic…
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Q: You use the Gram staining procedure to stain a bacterium that lacks a cell wall. Would you consider…
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Q: 400x 1000x What is the shape of bacteria under a microscope, what color is there, and is there Gram…
A: INTRODUCTION Gram staining is a differential staining used to differentiate bacteria into gram…
Q: What is Gram staining?Describe step by step the procedure.
A: GRAM STAINING METHOD:- This is a differential staining that was developed by Dr. Hans Christian Gram…
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A: Answer :- Subsequent to trying different things with presenting cells to gold and mercury, Golgi…
Q: Endospores can be seen as refractile structures in unstained cells and as colorless areas in…
A: Endospores are dormant, non-reproductive structures formed by certain bacteria during unfavorable…
Q: Why do we stain some organisms before viewing them under a microscope? (see top of p 14)
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Q: Janet is doing a Gram stain on a mixed culture that is known to contain both Gram positive and Gram…
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- Based from the results obtained from Gram staining, explain why bacterial cells retained or changed their color. Briefly explain the principle behind the procedure. Cite advantages of differential staining method (Gram staining and endospore staining) over simple staining only. List other staining methods aside from those you performed in the laboratory. conclusion:The bacterial cells in the image below were stained with a high concentration of carbolfuschin for an extended period of time (5 minutes) while the slide was heated. The slide was decolorized with a mixture of acid and alcohol. The slide was then counter-stained with methylene blue. What kind of stain does this describe?What is the principle of Grams staining based on the cell wall composition of bacterial cell.
- Endospores can be seen as refractile structures in unstained cells and as colorless areas in Gram-stained cells. Why is it necessary to do an endospore stain to verify the presence of endospores?What are the structural differences between Gram-positive and Gram-negative bacteria cell walls? Can you explain what would happen to the cell wall's physical integrity while applying the Gram staining technique?When doing a gram stain in microbiology, one step can be eliminated and still allow distinction between gram-positive and gram-negative organisms. What is the step and why would eliminating it still result in the ability to distinguish gram-positive from gram-negative?
- When performing a Gram stain on a Gram-negative bacteria, why does the bacteria stain pink? Crystal violet stain binds to iodine and remains in the bacterial cell wall because of the thick peptidoglycan layer. Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink. The alcohol wash removes crystal violet and iodine from the bacteria. Crystal violet stain binds to iodine and remains in the bacteria cell wall because of the thick peptidoglycan layer AND Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink. Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink AND The alcohol wash removes crystal violet and iodine from the bacteria.You are performing a Gram stain on Gram-negative bacteria, and you stop after the addition of the alcohol. What is the appearance of the bacteria at this point? ○ pink ○ colorless ○ blue ○ purpleWhy is it important to perform a gram stain for any type of bacteria? After performing the gram stain method what would be the next step? (Think in terms of the real-world situation) Explain.
- What is a step-by-step process of preparing and inspecting a microorganism under a microscope? Please provide an accurate answer.Describe in detail all the steps needed to determine whether an Unknown bacteria has a Gram-positive or Gram-negative cell wall using the Gram stain. (You can describe the method presented in the Exercise 3-6 Gram stain videos or in the Lab Manual.) 1. Start by describing the method of transferring the bacteria onto the microscope slide, then describe heat-fixing the emulsion of the Unknown bacteria. (Do not describe all the steps needed to perform aseptic technique. Just say “Bacteria was added aseptically.”) 2. Describe the Gram stain in detail. (This description should include all the stains, how long the stain should set on slide, and Genus and species names of bacteria you are using as CONTROLS.)Please discuss why staining is important in visualizing microbes under the microscope. What different types of staining methods can be used?