4th calculation: total amount (milligram) of DNA in 100g wheat germ assuming an extraction efficiency of 40%

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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please help with the 4th calculation: total amount (milligram) of DNA in 100g wheat germ assuming an extraction efficiency of 40%

You extract DNA from 200 milligram of wheat germ. Your total volume of DNA extraction
sample is 500 microlitres. You pipette 200 microlitres of this extraction sample into a 3-ml
cuvette and make up to 3.0 ml using buffer. The absorbance at 260 nm (Az60) of the solution in
the cuvette is 0.2 (Remember: an Az60 of 1.0 corresponds to a DNA concentration in the cuvette
solution of 50 micrograms per ml).
Calculate the total amount of DNA in 100g wheat germ, assuming that the extraction efficiency
of DNA from wheat germ during your lab protocol is 40%. Please fill out cach of the boxes in
the Table below
What to Calculate
| Calculation / Result
DNA concentration in
cuvette solution (in
microgram per ml)
Dilution factor of DNA
sample in cuvette
Total amount
(microgram) of DNA in
the entire extraction
sample
Total amount (milligram)
of DNA in 100g wheat
germ assuming an
extraction efficiency of
40%
In your extraction of DNA from wheat germ, would you expect that nuclear DNA is the only
source of DNA in your sample? Short answer (2 sentences max.)
Transcribed Image Text:You extract DNA from 200 milligram of wheat germ. Your total volume of DNA extraction sample is 500 microlitres. You pipette 200 microlitres of this extraction sample into a 3-ml cuvette and make up to 3.0 ml using buffer. The absorbance at 260 nm (Az60) of the solution in the cuvette is 0.2 (Remember: an Az60 of 1.0 corresponds to a DNA concentration in the cuvette solution of 50 micrograms per ml). Calculate the total amount of DNA in 100g wheat germ, assuming that the extraction efficiency of DNA from wheat germ during your lab protocol is 40%. Please fill out cach of the boxes in the Table below What to Calculate | Calculation / Result DNA concentration in cuvette solution (in microgram per ml) Dilution factor of DNA sample in cuvette Total amount (microgram) of DNA in the entire extraction sample Total amount (milligram) of DNA in 100g wheat germ assuming an extraction efficiency of 40% In your extraction of DNA from wheat germ, would you expect that nuclear DNA is the only source of DNA in your sample? Short answer (2 sentences max.)
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