Briefly explain Current Infection Marker (CIM) test, Campylobacter-Like Organism (CLO) test, and the multiplex polymerase chain reaction test (PCR)

Q: How real time RT-PCR can be used in detecting SARS-nCov2 virus? Please explain at your own words.

A: Reverse transcription- Polymerase chain reaction (RT-PCR) is a technique in which the RNA is…

Q: Explain how DNA microarrays are used in molecular profiling ofcancerous tumors.

A: DNA microarrays are microscopic slides that are printed with thousands of tiny spots in defined…

Q: The image above shows that, with respect to the WRN gene, the individual with Werner syndrome (WRN)…

A: Werner syndrome is a rare hereditary condition that causes early aging.It causes several health…

Q: Indirect ELISA can detect polygenic gene expression. Rewrite this

A: In polygenic gene inheritance some qualitative characters show discontinuous variation, but a…

Q: a. Explain whether or not DNA polymerase from a mesophilic bacterium could be used successfully in a…

A: A)- In a mesophile optimum condition would allow the microbe to replicate at it maximum amount…

Q: limitations of PCR viral diagnostic and explain how it could be improved

A: A significant biochemical technique that is used to generate multiple DNA (deoxyribonucleic acid)…

Q: PCR analysis of your sample did not work can you suggest why? How can you test if this is true?

A: PCR  stands for polymerase chain reaction    *PCR is nowdays used often in  hospitals and large…

Q: Comment on the failure of p1vir transduction experiments and give a reason why you think the…

A: transduction experiments is a technique commonly used in molecular biology to introduce foreign DNA…

Q: The PCB concentration in Lake Michigan is declining. The concentration can be modeled using a first…

A: The first order reaction is a reaction that proceeds at the rate that depends linearly on only one…

Q: Is this DNA extraction procedure needed/necessary for COVID19 screening? If not, what is its…

A: Because of the rapid spread and rising number of cases of coronavirus disease 19 (COVID-19),…

Q: How is plasmid DNA distinguished from coli chromosomal DNA using the Qiagen Miniprep protocol?

A: In the Qiagen Miniprep protocol, plasmid DNA is distinguished from E. coli chromosomal DNA based on…

Q: Suggest how the researchers could have altered their targeting construct, to ensure that all of the…

A: To ensure that all of the neomycin resistant transformants would have generated the expected PCR…

Q: ypes of PCR and how pcr works for SSR primers explain practical procedure and required apparatus

A: Polymerase Chain Reaction (PCR) is a molecular biology technique for duplicating a DNA sequence.…

Q: Currently I am working with qPCR quantification specific for Lactobacillus bacteria. I found in some…

A: The melting curves are generated when the DNA is heated along with the fluorescent DNA-binding dye.…

Q: List the major steps that would be required to clone the human insulin gene into a plasmid and to…

A: Gene cloning is the isolation of DNA (deoxyribonucleic acid) sequence or gene of interest and…

Q: What technique could be used to confirm the presence or absence of plasmids in the donor, recipient…

A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…

Q: write a detail note on base excision report? proper explanation and diagram

A: DNA repair is basically a collection of series of the different process through a cell can correct…

Q: PCR

A: Biotechnology: It is defined as the study of integration of natural and engineering sciences to…

Q: PCR is a useful tool for early diagnosis of an infectious disease.Elaborate.

A: A disease refers to an abnormal condition in the body that have negative impact on the function or…

Q: OTE: explain in a paragraph form How does PCR help in the identification of the virus from…

A: Polymerase chain reaction is a molecular biology technique for making a large number of copies of a…

Q: State the four steps of Microarray Anaylsis.

A: Using microarray analysis, it is possible to assess gene expression levels as well as recognise…

Q: Why is it important to incubate plates upside down (inverted)?

A: INTRODUCTION Why Petri plates are incubated upsidedown To avoid contamination.

Q: 2. PCR analysis practice One 1) If you collect samples from crime scene and extract DNA out of each…

A: For the amount of polymorphism in repeated sequences, any part of the DNA sample that is found in a…

Q: State the function of power supply, DNA marker, and ethidium bromide used in the electrophoresis…

A:

Q: Describe what is produced by a PCR assay, how it is detected, and explain how it can determine…

A: PCR, polymerase chain reaction, is the process of making many copies of DNA sequence in vitro. It is…

Q: It is possible to diagnose genetic mutation by PCR. Explain

A: The process of finding unknown or known mutations in the genes or genetic material is known as…

Q: How does diagnostic method for detecting Covid-19 using RT-PCR work?

A: RT–PCR also called Real-time PCR (polymerase chain reaction) is a nuclear-derived method or test…

Q: Order the following technical steps that are required to identify a bacterium by PCR and sequencing…

A: The objective of the question is to arrange the given steps in the correct order that are required…

Q: Give ONE suggestion for modifying the PCR reaction to detect other microbial  pathogens in the…

A: PCR tests used to detect disease when there is only a very small amount of pathogens is present in…

Q: sample containing at least one molecule of DNA serves as the starting material for PCR.  True…

A: PCR or polymerase chain reaction is routinely used in molecular biology laboratories that ultimately…

Q: Explain each section of the flowchart in detail by integrating all the concepts. The explanation…

A: The process by which identical copies of DNA (deoxyribonucleic acid) or fragments of DNA are…

Q: Place the stages of PCR in the correct order -Anneal -Denature -Analyse -Extend

A: Polymerase chain reaction (PCR) is a laboratory technique for rapidly amplifying DNA to produce…

Q: Please describe the role of each buffers (P1, P2, N3, PE, and EB) and RNAase A and Lyse blue during…

A: Role of P1 buffer - This buffer usually consists of a basic pH Tris buffer with EDTA…

Q: What is the nature of the primers used in RT-PCR tests? (i.e. are they DNA primers or RNA primers?)

A: In RT-PCR, the RNA sample obtained from a virus is converted into DNA, and the DNA is amplified and…

Q: You are assigned to analyze the genome of an unknown bacterium. Discuss three major steps that must…

A: Genome is the term used to define all the genetic information present in the body of an organism.…

Q: ELISA is a vital diagnostic tool. Reagents required for this technique include Thermostable DNA…

A: ELISA (Enzyme-linked immunosorbent assay) is a sensitive and specific laboratory technique used to…

Q: Tailed primer can have restriction enzyme sites. True False

A:

Q: a. What is the function of the SDS in the lysis buffer? the NaOH? b. What does the potassium…

A: These question are regarding plasmid DNA isolation

Pathogenicity

Infection and Transmission

The infections are generated by the pathogenic organisms present in the environment. They maintain the capacity to invade a host body and establish colonies. A disease caused by such infectious agents is called a communicable disease or transmissible disease. These diseases spread through diverse means including blood, food, water, air, or vectors.