Briefly explain Current Infection Marker (CIM) test, Campylobacter-Like Organism (CLO) test, and the multiplex polymerase chain reaction test (PCR)

Q: Evaluation of PCR product electrophoresed on 0.8% agarose gel shows non-specific bands. The…

A: Introduction DNA fingerprinting is a technique that is commonly used to identify people based on…

Q: Why is it important to read the product manual when designing your PCR conditions?

A: PCR (Polymerase Chain Reaction) is a laboratory technique used to amplify a specific DNA sequence…

Q: The three steps of a PCR reaction are Denaturation, polymerization and annealing Annealing,…

A: Scientists developed the polymerase chain reaction technology after combining a technique for…

Q: Give at least four(4) uses/application of PCR. Explain.

A: PCR (Polymerase Chain Reaction) is use to amplify DNA. It can make billions of copies of a target…

Q: If the RT-PCR test is negative, give instances when repeat RT-PCR test should be done. If repeated,…

A:

Q: What is southern blot? Understand PCR reaction in detail. What polymerase enzyme is used in PCR…

A: Both southern-blotting and PCR are types of recombinant DNA technology. Recombinant DNA technology…

Q: Indirect ELISA can detect polygenic gene expression. Rewrite this

A: In polygenic gene inheritance some qualitative characters show discontinuous variation, but a…

Q: a. Explain whether or not DNA polymerase from a mesophilic bacterium could be used successfully in a…

A: A)- In a mesophile optimum condition would allow the microbe to replicate at it maximum amount…

Q: limitations of PCR viral diagnostic and explain how it could be improved

A: A significant biochemical technique that is used to generate multiple DNA (deoxyribonucleic acid)…

Q: Differentiate Invitrogen pCR® II-TOPO® Vector from pBR322 Vector.

A: Molecular cloning : Production of genetically identical copies of a cell or its genetic material is…

Q: Explain the reason for the fact that lab should be scrupulously clean when performing the PCR by…

A: Introduction: PCR stands for Polymerase Chain Reaction. It is a technique used in molecular biology…

Q: The process of PCR essentially revolves around three phases.  Briefly describe these phases and the…

A: The polymerase chain reaction (PCR) is a widely used technique in molecular biology that allows…

Q: The primers used in PCR are double stranded DNA oligosaccharides. True False

A: The answer is False.  Primers are small, chemically synthesised oligonucleotides that are…

Q: How is plasmid DNA distinguished from coli chromosomal DNA using the Qiagen Miniprep protocol?

A: In the Qiagen Miniprep protocol, plasmid DNA is distinguished from E. coli chromosomal DNA based on…

Q: ypes of PCR and how pcr works for SSR primers explain practical procedure and required apparatus

A: Polymerase Chain Reaction (PCR) is a molecular biology technique for duplicating a DNA sequence.…

Q: What technique could be used to confirm the presence or absence of plasmids in the donor, recipient…

A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…

Q: Why is it necessary to chelate the metal ions from the solution during the boiling/lysis step at 100…

A: The polymerase chain reaction (PCR) is a fundamental biochemical method for exponentially…

Q: PCR technique is based on DNA transcription.  True  False

A: Introduction : PCR (polymerase chain reaction), the reaction mixture includes the DNA extract…

Q: PCR

A: Biotechnology: It is defined as the study of integration of natural and engineering sciences to…

Q: Why uncut and cut PCR at different position in the agarose gel? which one can be decided accuracy…

A: *Gel electrophoresis a technique to separate DNA fragments based on their size. *DNA samples are…

Q: Identify if the following statements are true or false. Quantitative PCR is also known as the…

A: A real-time polymerase chain reaction is a molecular genetics lab technology that involves the…

Q: PCR is a useful tool for early diagnosis of an infectious disease.Elaborate.

A: A disease refers to an abnormal condition in the body that have negative impact on the function or…

Q: Using the table below, what volume from the master mix will be dispensed to each sample tube if the…

A: Answer:  As we know, C1V1= C2V2, where C1 and C2 = initial and final concentrations V1 and V2 =…

Q: cite one reason why the RT-PCR test for COVID-19 detection may give a false negative result. assume…

A: RT PCR is reverse transcptase polymerase chain reaction used to determine the presence of virus in…

Q: What is cell lysis? Discuss the principles behind DNA isolation (extraction and precipitation…

A: A eukaryotic cell consists of many membrane-bound organelles. The membrane-bound organelles form…

Q: Biochemical and differential tests can be incorporated into growth media or performed on isolated…

A: It is required to provide three examples of differential test that is incorporated into growth media…

Q: Draw a diagram of what occurs in PCR during the annealing step of the FIRST cycle. b. Draw a diagram…

A: The PCR or polymerase chain reaction is a routinely used technique in molecular biology. It is used…

Q: Name four reagents used during a PCR reaction

A: Polymerase chain reaction: It is an in vitro technique that results in the exponential…

Q: Describe why different thermal conditions are necessary for successful PCR?

A: DNA polymerase is a natural polymerization enzyme that turns nucleotides into polynucleotide…

Q: Outline the method of real-time PCR, and discuss why it is used.

A: PCR which stands for polymerization chain reaction is an analytic technique which is used to amplify…

Q: Explain the concepts of excitation and emission in fluorescence in qRT-PCR

A: A real time polymerase chain reaction (continuous PCR) (Sometimes alluded to as qPCR) is a…

Q: Order the following technical steps that are required to identify a bacterium by PCR and sequencing…

A: The objective of the question is to arrange the given steps in the correct order that are required…

Q: Give ONE suggestion for modifying the PCR reaction to detect other microbial  pathogens in the…

A: PCR tests used to detect disease when there is only a very small amount of pathogens is present in…

Q: sample containing at least one molecule of DNA serves as the starting material for PCR.  True…

A: PCR or polymerase chain reaction is routinely used in molecular biology laboratories that ultimately…

Q: Explain each section of the flowchart in detail by integrating all the concepts. The explanation…

A: The process by which identical copies of DNA (deoxyribonucleic acid) or fragments of DNA are…

Q: Why is it important to wear gloves when setting up the PCR tubes?

A: PCR represents polymerase chain reaction which is an in vitro laboratory technique used for the…

Q: Place the stages of PCR in the correct order -Anneal -Denature -Analyse -Extend

A: Polymerase chain reaction (PCR) is a laboratory technique for rapidly amplifying DNA to produce…

Q: Please describe the role of each buffers (P1, P2, N3, PE, and EB) and RNAase A and Lyse blue during…

A: Role of P1 buffer - This buffer usually consists of a basic pH Tris buffer with EDTA…

Q: You are assigned to analyze the genome of an unknown bacterium. Discuss three major steps that must…

A: Genome is the term used to define all the genetic information present in the body of an organism.…

Q: Tailed primer can have restriction enzyme sites. True False

A:

Pathogenicity

Infection and Transmission

The infections are generated by the pathogenic organisms present in the environment. They maintain the capacity to invade a host body and establish colonies. A disease caused by such infectious agents is called a communicable disease or transmissible disease. These diseases spread through diverse means including blood, food, water, air, or vectors.